Korean J Gynecol Oncol.  2008 Mar;19(1):17-25. 10.3802/kjgo.2008.19.1.17.

Analysis of expression of survivin, caspase 3, and p53 protein in cervical neoplasia comparing with Ki-67 index

• Cha JY
• Cho TI
• Park TY
• So JS
• Choi SK
• Park ES

• KMID: 1979498
• DOI: http://doi.org/10.3802/kjgo.2008.19.1.17

Abstract

OBJECTIVE
The aim of this study was to determine the role of survivin, caspase 3, p53 and Ki-67 expression in the carcinogenesis of cervical carcinoma and aggressiveness of cervical intraepithelial neoplasia (CIN).
METHODS
The pathology specimens of 94 patients with a diagnosis of Low grade CIN (31 cases), High grade CINL (32 cases) and squamous cell carcinoma (31 cases) were evaluated immunohistochemically for the expression of survivin, caspase 3, p53 and Ki-67 in paraffin sections.
RESULTS
Survivin, p53 and Ki-67 expressions were progressively increased in accordance with the increasing degree of malignancy, but caspase 3 immunoreactivity was higher in high grade CIN than in low grade CIN and invasive cervical cancers. There was no significant difference between Ki-67 index and survivin, caspase 3 and p53 expression with the increasing degree of malignancy. The Ki-67 index was closely related to p53 overexpression in invasive cervical carcinoma group.
CONCLUSION
A sequential increase of survivin, p53, and Ki-67 was observed in paralleling the progression of grade of CIN and cervical cancer. In addition, caspase 3 expression increased proportionally to the low-grade CIN to high grade CIN.

Keyword

Survivin; Caspase 3; p53; Ki-67; Cervical neoplasia

MeSH Terms

Carcinoma, Squamous Cell
Caspase 3
Cervical Intraepithelial Neoplasia
Humans
Paraffin
Uterine Cervical Neoplasms
Caspase 3
Paraffin

Figure

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  Fig. 1 A low-grade cervical intraepithelial neoplasia showing survivin-positive cells scattered in the lower third of cervix (×200).

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  Fig. 2 A high-grade cervical intraepithelial neoplasia showing nuclear and cytoplasmic staining of caspase 3 (×200).

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  Fig. 3 Nuclear immunreactivity of p53 in a poorly differentiated invasive squamous cell carcinoma of cervix (×200).

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  Fig. 4 Immunostaining of Ki-67 showing nests of squamous cell carcinoma (×200).

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