J Korean Ophthalmol Soc.  1990 Mar;31(3):273-280.

The Change of T-lymphocyte Subsets in the Peripheral Blood of Herpes Simplex Keratitis Patients

• Kim SD
• Kim JS
• Kim JD

Abstract

This study was performed to investigate the change of T-lymphocyte subsets to evaluate the immune reactivity of herpes simplex keratitis patients. Peripheral blood lymphocytes were obtained from fourteen herpes simplex keratitis patients and fourteen controls. These cells were incubated with a panel of lymphocyte specific monoclonal antibodies and then CD4+ helper and CD8+ suppressor T cells were measured by the use of flow cytometer. The following results were obtained. 1. The mean age of the control group was 46.4+/-15.9(mean+/-SD)years. The proportion of CD4+ helper T cell was 41.93 +/- 7.89%(mean +/- SD), the proportion of CD8+ suppressor T cell was 24.35 +/- 5.54% and the CD4+ helper/ CD8+ suppressor T cell ratio was 1.86 +/- 0.47. 2. The mean age of herpes simples keratitis patients was 49.9 +/- 16.4 years. In the patients, CD4+ helper/ CD8+ supressor T cell ratio was 1.25 +/- 0.42, a decrease compared to cells from controls (P<0.01). The proportion of CD4+ helper T cell was 30.1 +/- 7.11%, a decrease compared to controls(P<0.01). CD8+ suppressor T cell was 25.96 +/- 7.90%. There was no significant change in the CD8+ suppressor T cell. In this study, herpes simplex keratitis patients were accompanied by decrement in the ratio of helper to suppressor T cells and this decreased ratio was due to a decrease in helper T cells. These results suggest that cell mediated immune reactivity is depressed in herpes simplex keratitis patients.

Keyword

Herpes simplex keratitis; helper and suppressor T-cell; flow cytometer; immune ractjvity

MeSH Terms

Antibodies, Monoclonal
Herpes Simplex*
Humans
Keratitis
Keratitis, Herpetic*
Lymphocytes
T-Lymphocyte Subsets*
T-Lymphocytes*
T-Lymphocytes, Helper-Inducer
Antibodies, Monoclonal