Substance P and VIP = induced Flare and Wheal Responses in Normal Healthy Persons

Ryu, byung Jick; Choi, Jee Ho; Koh, Jai Kyoung; Sung, Kyung Jeh

Abstract

BACKGROUND: Despite concern about information of neuropeptide, the has been no baseline study of neuropeptide in Koreans.
OBJECTIVE
The purpose of the study was to investigate the skin sinsitivity of substance P and VIP in normal healthy persoas.
METHODS
We prepared 1000pM, 100pM, 10pM solution of substan P 1-11, substnace P 1-7, substnace P 7-11, and VIP. We injected intradermally 50ul of the br ve solutions on 12 sites of both forearms in addition plaebo. We measured the size of the area of flare and wheal along time. We repeated the same test after antihistamine intake.
RESULTS
Flare and wheal respinses were dose dependent. Injection of substance P 1-7 did not evoke wheal responses and injection of substance P 7-11 did not wake flare responses. Flare responses of substance P 1-11, ubstance P 7-11, VIP were inhibiteb antihistamine and wheal responses of VIP were inhibitedly antihistamine.
CONCLUSION
N-terminal of subtance P is responsible for flarers onses and C-terminal of substnace P is responsible for wieal responses. Flare responses of sisance P were mediated by histamine but wheal responses osubstance P were direct effect on postcapillary venule. Flare and wheal responses of VIF were mediated by histamine.