Korean J Parasitol.  2014 Jun;52(3):243-249.

Lethal Effects of Helianthemum lippii (L.) on Acanthamoeba castellanii Cysts in Vitro

• Badria FA
• Hetta MH
• Sarhan RM
• Ezz El-Din MH

Abstract

Acanthamoeba spp. commonly cause Acanthamoeba keratitis which is typically associated with the wear of contact lenses. Therefore, finding an economic, efficient, and safe therapy of natural origin is of outmost importance. This study examined the in vitro lethal potential of ethyl acetate and methanol extracts of Helianthemum lippii (L.) (sun roses) against Acanthamoeba castellanii cysts isolated from patients with amoebic keratitis. Both extracts proved to be potent as regard to their lethal effects on A. castellanii cysts with comparable results to chlorhexidine. The ethyl acetate was more promising with cumulative lethality. It showed a highly significant lethal percentage along the duration of treatment. The analysis of the more potent ethyl acetate extract revealed the presence of 2.96 mg/100 g of total phenolics, 0.289 mg/100 ml of total flavonoids and 37 mg/100 mg of total tannins which highlighted their phytomedicinal role.

Keyword

Acanthamoeba castellanii; Helianthemum lippii; sun rose; ethyl acetate; methanol; keratitis

MeSH Terms

Acanthamoeba Keratitis/parasitology
Acanthamoeba castellanii/*drug effects/isolation & purification
Antiprotozoal Agents/isolation & purification/*pharmacology
Cell Survival/drug effects
Cistaceae/*chemistry
Flavonoids/isolation & purification
Humans
Parasitic Sensitivity Tests
Phenols/isolation & purification
Plant Extracts/isolation & purification/*pharmacology
Tannins/isolation & purification
Antiprotozoal Agents
Flavonoids
Phenols
Plant Extracts
Tannins