Korean J Vet Res.  2013 Dec;53(4):211-216.

Development and evaluation of protective capacity of Salmonella Enteritidis polyphosphate kinase-deleted and temperature-sensitive mutant

• Kim K
• Park S
• Cho Y
• Kwak JY
• Kang ZW
• Kim EH
• Choi HW
• Won HK
• Noh YH
• Hahn TW

Abstract

This study was focusing on evaluating the protection of polyphosphate kinase (ppk) deleted and/or temperature-sensitive (ts) Salmonella Enteritidis (SE) as an attenuated vaccine in chickens. We constructed SEppk, SEts and SEppk::ts mutants and screened those mutants by growth capability in vitro, protection study in mice model and antibody response in chickens. Among the mutants, SEppk::ts-3 was selected because it showed higher growth capability, good protection against highly virulent SE in mice model, and good antibody response in chickens. SEppk::ts-3 also showed good protection against highly virulent SE isolate because it decreased colonization of virulent SE challenge strain in spleen, liver and cecum compared with the non-vaccinated control. The SEppk::ts-3 mutant showed cross-protection against S. Gallinarum (SG) challenge although the its cross-protection rate was a little lower than that of SG9R, a commercial vaccine against SG infection. To use for live attenuated vaccine in chickens, it should further be characterized.

Keyword

cross protection; polyphosphate kinase deleted mutant; Salmonella Enteritidis; temperature-sensitive mutant

MeSH Terms

Animals
Antibody Formation
Cecum
Chickens
Colon
Cross Protection
Liver
Mice
Phosphotransferases
Salmonella enteritidis*
Salmonella*
Spleen
Phosphotransferases