J Vet Sci.  2014 Jun;15(2):241-248. 10.4142/jvs.2014.15.2.241.

Isolation, in vitro propagation, genetic analysis, and immunogenic characterization of an Ehrlichia canis strain from southeastern Brazil

  • 1Institute of Biomedical Sciences, Federal University of Uberlandia, Uberlandia 38405-320, Brazil. rnalves@icbim.ufu.br
  • 2Federal Institute of Education, Science and Technology of Triangulo Mineiro, Campus Uberlandia, Uberlandia 38400-974, Brazil.
  • 3Institute of Genetics and Biochemistry, Federal University of Uberlandia, Uberlandia 38400-902, Brazil.
  • 4Department of Preventive Veterinary Medicine and Animal Health, College of Veterinary Medicine, University of Sao Paulo, Sao Paulo 05508-270, Brazil.


Amplification of the 16S rRNA gene from a blood sample obtained from a dog in southeastern Brazil was used to confirm a naturally acquired Ehrlichia (E.) canis infection. Following isolation and culturing of the new bacterial strain called Uberlandia, partial sequences of the dsb and p28 genes were obtained. The dsb partial sequence of the novel strain was 100% similar to dsb gene sequences of E. canis obtained from different geographic areas around the world. Conversely, the p28 partial sequence for the E. canis Uberlandia strain differed at several nucleotides from other sequences available in GenBank. To confirm the antigenic profile of the Uberlandia strain, an indirect immunofluorescence assay against E. canis antigens was performed using dog sera collected from two different areas in Brazil (Uberlandia and Sao Paulo). The results suggest that both antigens were able to identify animals seropositive for E. canis in Brazil since these Brazilian strains appear to be highly conserved.


Brazil; Ehrlichia canis; genetic characterization; isolation; serology
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