J Korean Med Sci.  2009 Dec;24(6):1058-1063. 10.3346/jkms.2009.24.6.1058.

Use of Cytochrome c Oxidase Subunit I (COI) Nucleotide Sequences for Identification of the Korean Luciliinae Fly Species (Diptera: Calliphoridae) in Forensic Investigations

• Park SH
• Zhang Y
• Piao H
• Yu DH
• Jeong HJ
• Yoo GY
• Chung U
• Jo TH
• Hwang JJ

• KMID: 1783133
• DOI: http://doi.org/10.3346/jkms.2009.24.6.1058

Abstract

Blowflies, especially species belonging to the subfamily Luciliinae, are the first insects to lay eggs on corpses in Korea. Fast and accurate species identification has been a key task for forensic entomologists. Because conventional morphologic identification methods have many limitations with respect to forensic practice, molecular methods have been proposed to identify fly species of forensic importance. To this end, the authors amplified and sequenced the full length of the cytochrome c oxidase subunit I (COI) gene of the Luciliinae fly species collected in Korea. The results showed the COI sequences are instrumental in identifying Luciliinae fly species. However, when compared with previously reported data, considerable inconsistencies were noted. Hemipyrellia ligurriens data in this study differed significantly from two of the five pre-existing data. Two closely related species, Lucilia illustris and Lucilia caesar, showed an overlap of COI haplotypes due to four European sequences. The results suggest that more individuals from various geographic regions and additive nuclear DNA markers should be analyzed, and morphologic identification keys must be reconfirmed to overcome these inconsistencies.

Keyword

Forensic Medicine; Entomology; Diptera; Identification; Calliphoridae; Cytochrome-c Oxidase

MeSH Terms

Animals
*Base Sequence
Diptera/classification/enzymology/*genetics
Electron Transport Complex IV/*genetics
Forensic Medicine/*methods
Haplotypes
Humans
Korea
Molecular Sequence Data
Phylogeny
Postmortem Changes
Protein Subunits/*genetics
Sequence Analysis, DNA

Figure

• 

  Fig. 1 Phylogenetic analysis of Korean Luciliinae fly species. The letters Se, Am, Ca, Il, Li and Vi stand for Phaenicia sericata, Lucilia ampullacea, L. caesar, L. illustris, Hemipyrellia ligurriens, and Calliphora vicina, respectively. Calliphora vicina is included as the outgroup.

• 

  Fig. 2 Distribution of interspecific polymorphic sites on the COI gene from site 1 to 1,500.

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