Korean J Lab Med.  2007 Dec;27(6):406-413. 10.3343/kjlm.2007.27.6.406.

Proportions of Cells Expressing CD38-/CD34+, CD38+/CD34+, CD19+/CD34+, or CD13,33+/CD34+ in the Regenerating Bone Marrows During Complete Remission of Acute Leukemia or After Bone Marrow Transplantation

• Kahng J
• Shin SY
• Han K

• KMID: 1781544
• DOI: http://doi.org/10.3343/kjlm.2007.27.6.406

Abstract

BACKGROUND: The hemopoietic stem cells increase in number during the regeneration after chemotherapy or bone marrow transplantation (BMT). Although the proportion of hemopoietic stem cells and their differentiation have been studied by immunophenotyping using the flow cytometry, no substantial research efforts have been directed toward the regenerating marrow. We attempted to discover the proportions of undifferentiated stem cells, committed stem cells, B cell precursors, and myeloid precursors in the regenerating bone marrows during complete remission (CR) and after engraftment of BMT. METHODS: Bone marrow samples from 82 patients with acute leukemia in CR and from 25 patients after BMT engraftment, along with 22 control samples, were used to find the numbers of CD38-/CD34+, CD38+/CD34+, CD19+/CD34+, and CD13,33+/CD34+ cells in the large lymphocyte gate by flow cytometry. We cross-analyzed our results in terms of groups: CR, BMT, and initial diagnosis groups. We performed significance tests on age, relapse, chromosomal abnormalities, clinical outcomes, and initial immunophenotypes of the leukemic cells. RESULTS: The proportions of CD38-/CD34+, CD38+/CD34+, CD19+/CD34+, and CD13,33+/CD34+ cells are more highly distributed in acute B-lymphoblastic leukemia than the normal group and also in the CR than the BMT group. CD19+/CD34+ cells were increased in the relapse group and CD38+/ CD34+, CD19+/CD34+, and CD13,33+/CD34+ cells were increased in the group with chromosomal abnormality. The results were irrelevant to the initial immunophenotype of the leukemic blasts. CONCLUSIONS: The increases of the markers spanned too widely to apply one specific cutoff value to analyze them. They seemed to be the results of normal regeneration, irrelevant to relapse or initial immunophenotype of leukemic blasts.

Keyword

CD38-/CD34+; CD38+/CD34+; CD19+/CD34+; CD13,33+/CD34+; Regenerating bone marrow; Acute leukemia

MeSH Terms

Acute Disease
Antigens, CD19/*metabolism
Antigens, CD34/*metabolism
Antigens, CD38/*metabolism
Bone Marrow/physiology
*Bone Marrow Transplantation
Flow Cytometry
Follow-Up Studies
Granulocyte Colony-Stimulating Factor/therapeutic use
Hematopoietic Stem Cells/immunology/metabolism
Humans
Immunophenotyping
Leukemia/drug therapy/*metabolism/therapy
Regeneration
Remission Induction

Figure

• 

  Fig. 1. Dot plot scattergrams of bone marrow cells of a B-ALL patient in complete remission stained with anti-CD38-FITC/anti-CD34-PerCP (A, B). The large lymphocyte gate was set using forward scatter (FSC) and side scatter (SSC) characteristics (A). The CD38+/CD34+ cells in the gate (upper right quadrant) was 4.30% (B).

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