Ann Lab Med.  2012 Jan;32(1):87-90. 10.3343/alm.2012.32.1.87.

Chronic Pulmonary Disease Due to Mycobacterium monacense Infection: The First Case from Iran

• Shojaei H
• Hashemi A
• Heidarieh
• Hosseini N
• Daei Naser A

• KMID: 1781444
• DOI: http://doi.org/10.3343/alm.2012.32.1.87

Abstract

We herein report a case in which the recently characterized species Mycobacterium monacense was isolated from the sputum of an Iranian patient. This case represents the first isolation of M. monacense from Iran. The isolate was identified by conventional and molecular techniques. Our findings show that M. monacense infection is not restricted to developed countries.

Keyword

Mycobacterium; hsp65; 16S rDNA; rpoB gene; Ribosomal spacer

MeSH Terms

Bacterial Proteins/genetics
Chaperonin 60/genetics
Chronic Disease
Female
Humans
Iran
Lung Diseases/diagnosis/*microbiology
Middle Aged
Mycobacterium/classification/*genetics/isolation & purification
Mycobacterium Infections/*microbiology/pathology
Polymerase Chain Reaction
RNA, Ribosomal, 16S/genetics
Sputum/microbiology

Figure

• 

  Fig. 1 PCR restriction fragment length polymorphism patterns obtained from digestion of the amplified hsp65 gene of the isolate "M11" with HphI (A), HpaII (B) and AvaII (C), respectively. MW: the 50 bp molecular weight marker.

• 

  Fig. 2 Alignment of selected stretches of 16S rDNA gene of the Iranian isolate "M11" compared with those of closely related mycobacteria.

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