Ann Lab Med.  2014 Jan;34(1):15-19. 10.3343/alm.2014.34.1.15.

Comparison of ChromID Agar and Clostridium difficile Selective Agar for Effective Isolation of C. difficile from Stool Specimens

• Shin BM
• Lee EJ

• KMID: 1781354
• DOI: http://doi.org/10.3343/alm.2014.34.1.15

Abstract

BACKGROUND
ChromID Clostridium difficile agar (IDCd; bioMerieux SA, France) is a recently developed chromogenic medium for rapid and specific isolation of C. difficile. We compared the performance of IDCd with that of Clostridium difficile Selective Agar (CDSA).
METHODS
A total of 530 fresh stool specimens were collected from patients with clinical signs compatible with C. difficile infection, and cultures for C. difficile were performed on IDCd and CDSA. C. difficile colonies were identified by spore staining, odor, use of an ANI identification test kit (bioMerieux SA), and multiplex PCR for tcdA, tcdB, and tpi.
RESULTS
The concordance rate between IDCd and CDSA was 90.6% (480/530). The positivity rates on IDCd on days 1 and 2 (55.6% and 85.0%, respectively) were significantly higher than those on CDSA (19.4% and 75.6%, respectively) (P<0.001 for day 1 and P=0.02 for day 2), but the detection rates on IDCd and CDSA on day 3 were not different (89.4% vs. 82.8%, P=0.0914). On day 3, the recovery rates for non-C. difficile isolates on IDCd and CDSA were 30.2% (160/530) and 22.1% (117/530), respectively (P=0.0075). Clostridium spp. other than C. difficile were the most prevalent non-C. difficile isolates on both media.
CONCLUSIONS
The culture positivity rates on IDCd and CDSA were not different on day 3 but IDCd may allow for rapid and sensitive detection of C. difficile within 2 days of cultivation.

Keyword

Clostridium difficile; Culture; Chromogenic agar; Clostridium difficile selective agar

MeSH Terms

Agar/*chemistry
Bacterial Proteins/genetics
Bacterial Toxins/genetics
Clostridium difficile/genetics/*isolation & purification
DNA, Bacterial/analysis
Enterocolitis, Pseudomembranous/diagnosis/microbiology
Enterotoxins/genetics
Feces/*microbiology
Humans
Multiplex Polymerase Chain Reaction
Reagent Kits, Diagnostic
Triose-Phosphate Isomerase/genetics
Agar
Bacterial Proteins
Bacterial Toxins
DNA, Bacterial
Enterotoxins
Reagent Kits, Diagnostic
Triose-Phosphate Isomerase

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