Yonsei Med J.  2009 Jun;50(3):335-339. 10.3349/ymj.2009.50.3.335.

Metallo-beta-Lactamase-Producing Pseudomonas spp. in Korea: High Prevalence of Isolates with VIM-2 Type and Emergence of Isolates with IMP-1 Type

• Lee K
• Park AJ
• Kim MY
• Lee HJ
• Cho JH
• Kang JO
• Yong D
• Chong Y

• KMID: 1758559
• DOI: http://doi.org/10.3349/ymj.2009.50.3.335

Abstract

PURPOSE: Two Korean nationwide studies showed that metallo-beta-lactamases (MBLs)-producing-Pseudomonas spp. are not rare. The aim of this study was to assess the trends of MBL-producing isolates among imipenem-resistant isolates of Pseudomonas spp.
MATERIALS AND METHODS
Imipenem-resistant clinical isolates were collected from 23 hospitals and one commercial laboratory participating in the KONSAR program in 2005. Polymerase chain reaction (PCR) was used to detect MBL genes.
RESULTS
Alleles of MBL genes were detected in 10.8% of 415 Pseudomonas aeruginosa and 66.7% of 12 P. putida isolates from 18 of 24 hospitals/laboratory. Among the 14 IMP-1-like and 39 VIM-2-like MBLs, emergence of IMP-6 was detected for the first time.
CONCLUSION
Prevalence of MBL-producing P. aeruginosa has not significantly increased, but IMP-6 emerged in P. aeruginosa.

Keyword

IMP-1; IMP-6; VIM-2; metallo-beta-lactamase; Korea

MeSH Terms

Anti-Bacterial Agents/pharmacology
Electrophoresis, Gel, Pulsed-Field
Humans
Imipenem/pharmacology
Korea
Polymerase Chain Reaction
Pseudomonas Infections/*microbiology
Pseudomonas aeruginosa/drug effects/genetics/*metabolism
beta-Lactamases/genetics/*metabolism

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