J Korean Neurosurg Soc.  1993 Aug;22(8):877-883.

The Effect of Halothane-induced Acidosis on Focal Cerebral Ischemia in Rat

• Han KS
• Lee KC
• Chu JW
• Lee HK

Abstract

The current study was performed to investigate the influence of acidosis on focal cerebral ischemia in view of morphometric assay and neuropathological examination. The acidosis was induced by increment of halothane concentration and by decreasing respiratory rate. The mean pH were 7.423+/-0.012 in control group and 7.184+/-0.038 in acidosis group. Twenty-four hours after MCA occlusion(MCAO), neutral red staining and perfusion fixation was performed. The ischemic area was measured and morphometric analysis was undertaken. In acidosis group, the infarct area was 25.23+/-4.78% of the total cerebral area;in control group, the infarct area was 27.69+/-4.05%. The histopathological findings were examined under light microscopy, in which the field scanning was carried out from the midline by 0.5mm interval at cortical and basal ganglia levels. These results indicated that although there was no satistically significant difference in infarct area between acidosis and control group, increased acidosis aggravated the extent of histopathologic ischemic neuronal damage.

Keyword

Acidosis; Focal cerebral ischemia; Morphometric assay histopathologic examination

MeSH Terms

Acidosis*
Animals
Basal Ganglia
Brain Ischemia*
Halothane
Hydrogen-Ion Concentration
Microscopy
Neurons
Neutral Red
Perfusion
Rats*
Respiratory Rate
Halothane
Neutral Red