Korean J Dermatol.  1981 Aug;19(4):385-389.

Adenosine Deaminase in Human Skin

• Kim YP
• Kahng JB
• Chon JY
• Ihm CW

Abstract

Adenosine deaminase (adenosine aminohydrolase, AL)A), which catalyzes the deamination of adenosine to yield inosine and amrnonia, was assayed in human penile foreskin. The skin tissue was separated into two layers; epidermis and dermis. They were sliced with scissors into gel state, 4 volumes of 0.05M phosphate buffer solution were added and the tissue homogenized. After centrifugation at 4,000xg for 5 minutes, the supernatant was used as an enzyme solution. ADA activity was measured according to the method f Giuseppe" ADA was found to be present in both layers (epidermis; 0.24 OD/mg protein, dermis; 0.19 OD/mg protein) with slightly higher activity in the epidermis. As in earlier reports, it was found that ADA in the skin showed nearly even activity in the pH range of 5.0-8.0. Considering the significance of ADA in immunological function, the presence of ADA in the skin suggests that the tissue may participate in the immune function.

MeSH Terms

Adenosine Deaminase*
Adenosine*
Centrifugation
Deamination
Dermis
Epidermis
Foreskin
Humans*
Hydrogen-Ion Concentration
Inosine
Skin*
Adenosine
Adenosine Deaminase
Inosine