J Bacteriol Virol.  2005 Dec;35(4):291-298.

Rapid Serological Identification for Monophasic Salmonella Serovars with a hin Gene: Specific Polymerase Chain Reaction

Affiliations
  • 1Division of Bacterial Enteric Infections, Center for Infectious Diseases, National Institute of Health, Seoul, Korea. bokrates@nih.go.kr

Abstract

Salmonellosis is one of the most common food born diseases in Korea. However, it takes more than 8 days and many expensive antiserums are used for the identification of Salmonella serovars since the microorganism easily undergoes phase variation. According to the data that 65.5% of Salmonella isolates in 2000~2004 year had monophasic flagella, we have developed a rapid serological identification method using a hin gene-specific polymerase chain reaction (PCR) for monophasic Salmonella isolates that does not require the time-consuming phase conversion experiments. Using our new method, 'hin specific PCR-based serological test', we could identify serovars of monophasic Salmonella in 4 days. For the purpose of rapid identification of salmonella serovars collected from outbreaks and sporadic cases, hin specific PCR-based serological tests will be a fast and efficient method.

Keyword

Monophasic Salmonella; hin; PCR; Serological identification
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