Korean J Parasitol.  2013 Apr;51(2):255-258. 10.3347/kjp.2013.51.2.255.

In Vitro Effects of SB202190 on Echinococcus granulosus

• Lv H
• Li S
• Zhang J
• Liang W
• Mu X
• Jiang Y

• KMID: 1499708
• DOI: http://doi.org/10.3347/kjp.2013.51.2.255

Abstract

Spillage of cyst contents during surgical operation is the major cause of recurrence after hydatid cyst surgery. Instillation of a scolicidal agent into a hepatic hydatid cyst is the most commonly employed measure to prevent this complication. SB202190 is a pyridinyl imidazole derivative and is known to be a specific inhibitor of p38 MAPK. In the present study, the scolicidal effect of SB202190 was investigated. Freshly isolated Echinococcus granulosus protoscolices were subjected to SB202190 treatment (10, 20, 40, and 80 microM), and the effects on parasite viability were monitored by trypan blue staining. Corresponding effects were visualized by scanning and transmission electron microscopy. Dose-dependent protoscolex death within a few days of SB202190 treatment was observed. Although the in vitro scolicidal effect of SB202190 was satisfactory, the in vivo efficacy of this drug and also possible side effects remain to be further investigated.

Keyword

Echinococcus granulosus; hydatid cyst; protoscolex; SB202190

MeSH Terms

Animals
Anthelmintics/*pharmacology
Dose-Response Relationship, Drug
Echinococcus granulosus/*drug effects/ultrastructure
Imidazoles/*pharmacology
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Parasitic Sensitivity Tests
Pyridines/*pharmacology
Survival Analysis
Anthelmintics
Imidazoles
Pyridines