J Bacteriol Virol.  2008 Sep;38(3):139-147. 10.4167/jbv.2008.38.3.139.

Detection of Infectious Bursal Disease Virus by Double Antibody Sandwich ELISA

• Jeon WJ
• Chang BS
• Park MJ
• Lee EK
• Joo HD
• Kwon JH
• Choi KS

• KMID: 1483972
• DOI: http://doi.org/10.4167/jbv.2008.38.3.139

Abstract

Infectious bursal disease virus (IBDV) is responsible for a highly contagious disease of poultry causing severe immunosuppression in chickens. A double antibody sandwich ELISA (DAS-ELISA) was developed to detect IBDV from clinical samples. Two kinds of anti-IBDV antibodies, monoclonal antibody R63 and chicken anti-IBDV sera, were used for DAS-ELISA. Detection limit of IBDV by DAS-ELISA was approximately 10(2.7) EID(50)/ml. The DAS-ELISA detected IBDV from most (13/14) of vaccine products including mild, intermediate and intermediate-plus types. The DAS-ELISA also detected IBDV from all (19/19) of field Korean isolates including very virulent and intermediate-plus phenotypes. Our results indicate that the DAS-ELISA would provide useful diagnostic tool to detect IBDV from clinical samples as well as rapid quantitative detection of IBDV.

Keyword

Antigen detection; Diagnosis; ELISA; Infectious bursal disease

MeSH Terms

Antibodies, Monoclonal
Chickens
Enzyme-Linked Immunosorbent Assay
Immunosuppression
Infectious bursal disease virus
Limit of Detection
Phenotype
Poultry
Viruses
Antibodies, Monoclonal

Figure

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  Figure 1. Quantitative titration of IBDV D67 strain by DAS ELISA (B) and comparison with RT-PCR assay (A).

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  Figure 2. Detection of IBDV in commercial IBDV vaccines by DAS-ELISA (B) and comparison with results of RT-PCR (A). Data in the RT-PCR (A) represents relative quantity percent of each amplified DNA to total DNA amplified in the gel.

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  Figure 3. Detection of field isolates of IBDV by DAS-ELISA. Line (OD 0.53 at 450 nm) represents cut-off.

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