J Korean Orthop Res Soc.  2011 Dec;14(2):33-41.

Comparison of Cytocompatibility Between Grit Blasted Titanium Alloy (Ti-6Al-4V) with or without Pure Titanium Coating

• Kim J
• Lim YW
• Kwon SY
• Kim YS

Abstract

PURPOSE
A composite of aluminum and vanadium (Ti-6Al-4V) is one of the most common compositions of titanium-based alloys. Unfortunately, vanadium has been found to cause adverse reactions. We evaluated the effects of vanadium containing titanium alloy (Ti-6Al-4V) on an osteoblast-like cell line (SaOS-2).
MATERIALS AND METHODS
We studied the biologic and morphologic responses of SaOS-2 cell to Ti alloy with grit blasting and Ti coated Ti alloy with grit blasting. We performed energy-dispersive x-ray spectroscopy (EDS) and scanning electron microscopy (SEM) investigations and performed a cell proliferation assay, ALP activity, and cell migration assay of SaOS-2 cells.
RESULTS
The morphologic assessment of cells through SEM showed that the two surfaces were covered with similar amounts of small slender osteoblast like cells. The amount of proliferation, ALP activity and the migration extent of SaOS-2 cells on the surfaces of each group were not statistically different.
CONCLUSION
We used a grit-blasted Ti-coated Ti alloy, coated using electron beam deposition, and a grit-blasted Ti alloy to evaluate the toxicity of Ti-6Al-4V on SaOS-2 cell. Compared with pure titanium, the vanadium-containing Ti-alloy did not show an adverse effect on SaOS-2 cells.

Keyword

Ti alloy; Pure titanium; Electron beam deposition; Cytocompatibility

MeSH Terms

Alloys
Aluminum
Cell Line
Cell Migration Assays
Cell Proliferation
Electrons
Microscopy, Electron, Scanning
Osteoblasts
Spectrum Analysis
Titanium
Vanadium
Alloys
Aluminum
Titanium
Vanadium