J Vet Sci.
2011 Dec;12(4):405-407. 10.4142/jvs.2011.12.4.405.
Post-mortem re-cloning of a transgenic red fluorescent protein dog
- Affiliations
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- 1Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. bclee@snu.ac.kr
- KMID: 1365026
- DOI: http://doi.org/10.4142/jvs.2011.12.4.405
Abstract
- Recently, the world's first transgenic dogs were produced by somatic cell nuclear transfer. However, cellular senescence is a major limiting factor for producing more advanced transgenic dogs. To overcome this obstacle, we rejuvenated transgenic cells using a re-cloning technique. Fibroblasts from post-mortem red fluorescent protein (RFP) dog were reconstructed with in vivo matured oocytes and transferred into 10 surrogate dogs. One puppy was produced and confirmed as a re-cloned dog. Although the puppy was lost during birth, we successfully established a rejuvenated fibroblast cell line from this animal. The cell line was found to stably express RFP and is ready for additional genetic modification.
MeSH Terms
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Animals
Animals, Genetically Modified
Cloning, Organism/methods/*veterinary
Dogs/*genetics
Female
Gastrointestinal Tract/metabolism
Gene Expression Regulation
Kidney/metabolism
Liver/metabolism
Luminescent Proteins/*genetics/metabolism
Lung/metabolism
Male
Myocardium/metabolism
Nuclear Transfer Techniques/veterinary
Spleen/metabolism
Trachea/metabolism
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