The Effect of Administration of Cyclosporin A in Lipopolysaccharide Induced Otitis Media Model

Bang, Choong Ill; Yeo, Sang Won

Abstract

BACKGROUND AND OBJECTIVES
It has been known that immune reaction plays important roles in the pathogenesis of otitis media with effusion (OME). However, the immunologic mechanisms of OME have not been well defined yet. We investigated the changes of middle ear mucosal inflammation induced by lipopolysaccharide (LPS) following administration of immunosuppressive agent, Cyclosporin A (CsA) in experimental animals. MATERIALS AND METHOD: 35 mul LPS was injected into the middle ears of 30 rats. These rats were divided into groups of 10, each receiving CsA, Dexamethasone, and no treatment, respectively, daily. Five rats from each group were sacrificed at day 2 and 6 after the injection of LPS. RESULTS: Both the CsA-treated and the steroid-treated groups showed lesser inflammatory changes than the untreated groups, however, there were no significant differences between the CsA-treated and the steroid-treated groups. Both on day 2 & day 6, the CsA-treated group showed a decrease of mucosal metaplasia and secretory epithelium, compared with the untreated and steroid-treated group. CONCLUSION: The results suggest that CsA may be effective in decreasing inflammation with particular significance in mucosal mataplasia and the secretion of effusion in OME.