Exp Mol Med.  2003 Aug;35(4):310-316.

Detection of antibodies against glucose 6-phosphate isomerase in synovial fluid of rheumatoid arthritis using surface plasmon resonance (BIAcore)

  • 1Department of Pathology and Center for Molecular Medicine, Samsung Biomedical Research Institute, School of Medicine, Sungkyunkwan University, Suwon 440-746, Korea. tjkim@med.skku.ac.kr
  • 2Department of Medicine, Samsung Medical Center, School of Medicine, Sungkyunkwan University, Seoul 135-710, Korea.


We have used a surface plasmon resonance biosensor (SPR, BIACORE 2000) to detect antibodies against glucose 6-phosphate isomerase (GPI) in synovial fluids of rheumatoid arthritis (RA) and osteoarthritis (OA). Recombinant human GPI proteins fused with or without NusA were expressed in E. coli, purified to homogeneity and immobilized in flow cells of CM5 sensor chips. The flow cells immobilized with NusA protein or bovine serum albumin were used to monitor non-specific binding. Synovial fluid samples from RA patients showed a significantly higher level of binding to recombinant GPI proteins than samples from OA patients. Proteins which bound to the recombinant GPI proteins were confirmed to be immunoglobulin through the administration of anti-human immunoglobulin. NusA fusion protein was excellent for this assay because of a low background binding activity in the SPR analysis and its advantage of increased solubility in recombinant protein production. These results suggested a useful utilization of recombinant NusA-GPI fusion protein for the detection of autoantibodies against GPI in RA patients.


autoimmune disease; biosensing techniques; glucose 6-phosphate isomerase; rheumatoid arthritis; Serology

MeSH Terms

Arthritis, Rheumatoid/*immunology
Glucose-6-Phosphate Isomerase/genetics/*immunology/metabolism
Middle Aged
Peptide Elongation Factors/genetics/metabolism
Recombinant Fusion Proteins/genetics/metabolism
Surface Plasmon Resonance
Synovial Fluid/*immunology
Transcription Factors/genetics/metabolism
Full Text Links
  • EMM
export Copy
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr