J Korean Med Sci.  1998 Feb;13(1):21-26. 10.3346/jkms.1998.13.1.21.

Immunohistochemical characterization of the cellular infiltrate in airway mucosa of toluene diisocyanate (TDI)-induced asthma: comparison with allergic asthma

• Park HS
• Hwang SC
• Nahm DH
• Yim HE

• KMID: 1107221
• DOI: http://doi.org/10.3346/jkms.1998.13.1.21

Abstract

Toluene diisocyanate (TDI) is the most prevalent agent in occupational asthma (OA) in Korea. The immuno-pathologic mechanism for TDI-induced bronchoconstriction remains to be clarified. We studied the immunohistochemical finding of inflammatory cells in bronchial mucosa in subjects with TDI-induced asthma. Fiberoptic bronchial biopsy specimens were obtained from nine subjects with TDI-induced asthma. Six allergic asthma sensitive to house dust mite were enrolled as controls. Bronchial biopsy specimens were examined by immunohistology with a panel of monoclonal antibodies to mast cell tryptase (AA1), secretary form of eosinophil cationic protein (EG2), pan T-lymphocyte (CD3) and neutrophil elastase (NE). There was a significant increase in the number of AA1+, EG2+ and NE+ cells in TDI-induced asthma compared to those of allergic asthma (p=0.02, p=0.04, p=0.03, respectively). No significant differences were observed in the number of CD3+ cells (p=0.27). These findings support the view that neutrophil recruitment together with eosinophil and mast cell, may contribute to the bronchoconstriction induced by TDI.

MeSH Terms

Adult
Asthma/pathology
Asthma/immunology*
Asthma/chemically induced
Bronchi/pathology
Bronchi/immunology*
Bronchi/cytology
Eosinophils/immunology
Eosinophils/cytology
Female
Human
Leukocyte Count
Male
Mast Cells/immunology
Mast Cells/cytology
Middle Age
Mucous Membrane/immunology
Neutrophils/immunology
Neutrophils/cytology
T-Lymphocytes/immunology
T-Lymphocytes/cytology
Toluene 2,4-Diisocyanate/adverse effects*