J Vet Sci.  2007 Dec;8(4):329-333. 10.4142/jvs.2007.8.4.329.

Immunolocalization of anion exchanger 1 (Band 3) in the renal collecting duct of the common marmoset

• Song JH
• Kim YH
• Kang TC
• Won MH
• Suh JG
• Hyun BH
• Oh YS
• Ryu SY
• Jung JY

• KMID: 1106211
• DOI: http://doi.org/10.4142/jvs.2007.8.4.329

Abstract

The purpose of this study was to determine the expression and distribution of band 3 in the collecting duct and connecting tubules of the kidney of the marmoset monkey (Callithrix jacchus), and to establish whether band 3 is expressed in type A intercalated cells. The intracellular localization of band 3 in the different populations of intercalated cells was determined by double-labeling immunohistochemistry. Immunohistochemical microscopy demonstrated that band 3 is located in the basolateral plasma membranes of all type A intercalated cells in the connecting tubule (CNT), cortical collecting duct (CCD), and outer medullary collecting duct (OMCD) of the marmoset. However, type B intercalated cells and non-A/ non-B intercalated cells did not show band 3 labeling. Electron microscopy of the CNT, CCD and OMCD confirmed the light microscopic observation of the basolateral plasma membrane staining for band 3 in a subpopulation of interacted cells. Basolateral staining was seen on the plasma membrane and small coated vesicles in the perinuclear structure, some of which were located in the Golgi region. In addition, there was no labeling of band 3 in the mitochondria of the CNT, CCD and in OMCD cells. The intensity of the immunostaining of the basolateral membrane was less in the CNT than in the CCD and OMCD. In contrast, band 3 immunoreactivity was greater in the intracellular vesicles of the CNT. From these results, we suggest that the basolateral Cl-/HCO3- exchanger in the monkey kidney is in a more active state in the collecting duct than in the CNT.

Keyword

band 3 protein; kidney; marmoset; monkey

MeSH Terms

Animals
Anion Exchange Protein 1, Erythrocyte/*metabolism
Callithrix/*metabolism
Gene Expression Profiling/veterinary
*Gene Expression Regulation
Immunohistochemistry/veterinary
Kidney Tubules/cytology/physiology/ultrastructure
Kidney Tubules, Collecting/cytology/*metabolism/ultrastructure
Male
Microscopy, Electron, Transmission/veterinary

Figure

• 

  Fig. 1 Light micrographs of 50 µm thick vibratome sections showing immunostaining for band 3 in the cortex (A, C) and outer medulla (B, D) of the marmoset kidney. A: In the cortex of the marmoset kidney, band 3 immunoreactivity was evident in the connecting tubule (CNT) and cortical collecting duct (CCD). B: In the outer medulla of the marmoset monkey kidney, band 3 immunoreactivity was evident in the outer medullary collecting duct (OMCD). C, D: Higher magnification of A and B, respectively. Scale bar = 100 µm.

• 

  Fig. 2 Differential interference contrast (DIC) microscopy of 1 µm sections of the CNT and CCD (A) and OMCD (B) of the marmoset kidney. Double immunolabeling for H+-ATPase (blue) and band 3 (brown) in 1 µm sections shows that band 3 is expressed in the basolateral plasma membrane of H+-ATPase-positive intercalated cells. Arrowheads, type A intercalated cells with basolateral band 3 and apical H+-ATPase in the CNT, CCD, and OMCD. Arrows, type B intercalated cells with basolateral H+-ATPase in the CNT and CCD. Stars, non-A/non-B cells with apical H+-ATPase in the CNT and CCD. Scale bar = 100 µm.

• 

  Fig. 3 Transmission electron micrographs showing band 3 immunoreactivity in the CNT (A), CCD (B), and OMCD (C) of the marmoset kidney. Band 3 immunoreactivity is evident along the basolateral membrane and in vesicles in the basolateral parts of the cytoplasm (A-C). The intensity of the band 3 immunoreactivity of the basolateral plasma membrane in the CCD and OMCD cells is stronger than that in the CNT cells in A. Scale bar = 2.5 µm.

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