J Vet Sci.  2007 Mar;8(1):81-87. 10.4142/jvs.2007.8.1.81.

Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos

• Song K
• Lee E

• KMID: 1104240
• DOI: http://doi.org/10.4142/jvs.2007.8.1.81

Abstract

This study examined effects on the developmental competence of pig oocytes after somatic cell nuclear transfer (SCNT) or parthenogenetic activation (PA) of : 1) co-culturing of oocytes with follicular shell pieces (FSP) during in vitro maturation (IVM); 2) different durations of maturation; and 3) defined maturation medium supplemented with polyvinyl alcohol (PVA; control), pig follicular fluid (pFF), cysteamine (CYS), or beta-mercaptoethanol (beta-ME). The proportion of metaphase II oocytes was increased (p < 0.05) by co-culturing with FSP compared to control oocytes (98% vs. 94%). However, blastocyst formation after SCNT was not improved by FSP coculture (9% vs. 12%). Nuclear maturation of oocytes matured for 39 or 42 h was higher (p < 0.05) than that of oocytes matured for 36 h (95-96% vs. 79%). Cleavage (83%) and blastocyst formation (26%) were significantly higher (p < 0.05) in oocytes matured for 42 h than in other groups. Supplementation of a defined maturation medium with 100 micrometer CYS or 100 micrometer beta-ME showed no stimulatory effect on oocyte maturation, embryo cleavage, or blastocyst formation after PA. beta-ME treatment during IVM decreased embryo cleavage after SCNT compared to pFF or PVA treatments, but no significant difference was found in blastocyst formation (7-16%) among the four treatment groups. The results indicated that maturation of oocytes for 42 h was beneficial for the development of SCNT embryos. Furthermore, the defined maturation system used in this study could support in vitro development of PA or SCNT embryos.

Keyword

embryo development; oocyte maturation; parthenogenesis; pig; somatic cell nuclear transfer

MeSH Terms

Animals
Cysteamine
Embryo Culture Techniques/*veterinary
Embryo, Mammalian/*physiology
Female
Follicular Fluid
Mercaptoethanol
Nuclear Transfer Techniques/*veterinary
Oocytes/*growth & development
Sus scrofa/*physiology
Time Factors

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