J Vet Sci.
2002 Jun;3(2):61-66.
Assessment of Replication and Virulence of Attenuated Pseudorabies Virus in Swine
- Affiliations
-
- 1Animal Health Group, Pfizer Inc, Lincoln, Nebraska USA.
- 2Veterinary Resources, Inc., Ames, Iowa USA.
- 3Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, University of Iowa, Ames, Iowa USA.
- 4Department of Avian Medicine, College of Veterinary Medicine, University of Georgia, Athens, Georgia USA.
- 5Animal Health Group, Pfizer Inc, New York, New York, USA. tom.newby@pfizer.com
Abstract
- A nonclinical study was conducted to characterize the replication behavior of a modified live gE-deleted pseudorabies virus (PRV MS) in swine and potential for reversion to virulence after animal passages. Two to 3 week-old weaned pigs, negative for PRV, were maintained in isolation and challenged by intranasal instillation. For the first passage, 6 pigs were given 1 mL of PRV MS (107.3 TCID 50/mL) and 2 were necropsied at 3, 4 and 5 days post-inoculation (PI). Brain and secondary lymphoid tissues were collected, homogenized and the supernatants individually pooled for virus isolation, and PRV was recovered from each sample. No clinical signs of PRV infection were observed, but each pig had a nasal swab suspect or positive for PRV. For the second passage, 5 pigs were given 1 mL of the homogenate of mixed tissues from 1 animal in the previous passage (PRV at 101.9 TCID 50/mL). At 5 days PI, all pigs were necropsied, and PRV was not recovered from their tissue homogenates or nasal swabs, and no clinical signs were observed. During a second attempt at a second passage, tissue homogenates from all pigs in the first passage (PRV at approximately 101.7 TCID50 50/mL) were pooled and used to inoculate 15 pigs with 2 mL for 3 consecutive days. Ten pigs were monitored for clinical signs and seroconversion through 21 days PI, and 5 pigs were necropsied at 5 days PI. No clinical signs or PRV antibodies were detected in the 10 monitored pigs, and no PRV was recovered from the homogenates or nasal swabs of the 5 necropsied pigs. Thus, no evidence of reversion to virulence was demonstrated in pigs given the attenuated PRV.
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