J Vet Sci.  2006 Dec;7(4):349-354. 10.4142/jvs.2006.7.4.349.

Analysis of the seroprevalence of bovine paratuberculosis and the application of modified absorbed ELISA to field sample testing in Korea

Affiliations
  • 1Department of Microbiology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. yhp@snu.ac.kr
  • 2KRF Zoonotic Disease Priority Research Institute, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
  • 3Department of Bacteriology, National Veterinary Research and Quarantine Service, Anyang 430-824, Korea.
  • 4Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USA.

Abstract

Paratuberculosis (PTB) is a major disease problem worldwide, and causes major economic losses in the dairy industry. Although PTB has been reported in Korea, no studies have been conducted to determine its prevalence and no program has been developed to control the disease. In this study, the sera of beef (n = 1,056) and dairy cattle (n = 1,105) from all provinces in Korea were tested to determine the prevalence of PTB using two different ELISA: an 'in house' modified absorbed ELISA (P-ELISA) based on sonicated antigen from Mycobacterium avium subsp. paratuberculosis ATCC 19698, and a commercial ELISA (C-ELISA). Receiver operating characteristic analysis was used to determine the cutoff point for P-ELISA. Based on C-ELISA results, the area under the curve for P-ELISA was 0.913 (95% CI, 0.883 to 0.943). Using a cutoff point of 0.100, P-ELISA showed a sensitivity of 62.0% and a specificity of 93.7%. The kappa value and the percent agreement between the two ELISAs were 0.322 and 92.5%, respectively. Both ELISAs showed a significant correlation between age and seropositivity (p < 0.01). According to C-ELISA, 71 of 2,161 sera (3.3%, 95 CI, 2.6% to 4.1%) were test-positive. The national true prevalence of PTB was estimated to be 7.1%. The findings suggest that a control program should be implemented to limit the spread of this disease, and that P-ELISA could be used as a screening test that produces results similar to C-ELISA.

Keyword

absorbed ELISA; Mycobacterium paratuberculosis; paratuberculosis; prevalence
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