J Vet Sci.  2009 Sep;10(3):177-180. 10.4142/jvs.2009.10.3.177.

Immunohistochemical localization of galectin-3 in the granulomatous lesions of paratuberculosis-infected bovine intestine

Affiliations
  • 1Department of Veterinary Anatomy, College of Veterinary Medicine and Applied Radiological Science Research Institute, Jeju National University, Jeju 690-756, Korea. shint@cheju.ac.kr
  • 2Department of Veterinary Anatomy, College of Veterinary Medicine and Animal Medical Institute, Chonnam National University, Gwangju 500-757, Korea.
  • 3Department of Microbiology, College of Medicine, Jeju National University, Jeju 690-756, Korea.

Abstract

The presence of galectin-3 was immunohistochemically quantified in bovine intestines infected with paratuberculosis (Johne's disease) to determine whether galectin-3 was involved in the formation of granulation tissue associated with the disease. Mycobacterium avium subsp. paratuberculosis infection was histochemically confirmed using Ziehl-Neelsen staining and molecularly diagnosed through rpoB DNA sequencing. Galectin-3 was detected in the majority of inflammatory cells, possibly macrophages, in the granulomatous lesions within affected tissues, including the ileum. These findings suggest that galectin-3 is associated with the formation of chronic granulation tissues in bovine paratuberculosis, probably through cell adhesion and anti-apoptosis mechanisms.

Keyword

bovine paratuberculosis; galectin-3; granulation; intestine

MeSH Terms

Animals
Cattle
Cattle Diseases/*pathology
Chronic Disease
Galectin 3/*metabolism
Immunohistochemistry
Intestine, Small/microbiology/*pathology
Mycobacterium avium subsp. paratuberculosis/growth & development/isolation & purification
Paratuberculosis/*pathology
RNA Polymerase II/genetics
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