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Correlations between embryo morphokinetic development and maternal age: Results from an intracytoplasmic sperm injection program

Faramarzi , Khalili MA, Mangoli E

OBJECTIVE: It is widely accepted that aging decreases women’s fertility capacity. The aim of this study was to assess correlations between maternal age and the morphokinetic parameters and cleavage pattern...
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Male Inflammatory Parameters Are not Useful to Predict the Outcomes of Intracytoplasmic Sperm Injection: Results from a Cross-Sectional Study

Cito G, Coccia ME, Picone R, Cocci A, Russo GI, Cai T, Bencini G, Fucci R, Micelli E, Criscuoli , Bertocci F, Borrani E, Serni S, Carini M, Natali A

PURPOSE: The relationship between male systemic inflammation and fertility seems intriguing, but no data about its impact on the assisted reproductive technology outcomes has been reported. Here, we aimed to...
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Effect of Sperm DNA Fragmentation on Embryo Quality in Normal Responder Women in In Vitro Fertilization and Intracytoplasmic Sperm Injection

Kim SM, Kim SK, Jee BC, Kim SH

PURPOSE: To investigate the associations between sperm DNA fragmentation (SDF) and embryo formation rate in normal responder women to in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI). MATERIALS AND METHODS: Fifty-three consecutive, fresh...
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A point of confusion for embryologists in the identification of viable spermatozoa by the eosin-nigrosin test

Chen H, Zhou H, Shu J, Gan X, Wang C, Lin R

A viable spermatozoon is a prerequisite for fertilization in intracytoplasmic sperm injection (ICSI). Thus, it is crucial to select viable but immotile spermatozoa on the day of ICSI. We report...
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Growth differentiation factor 9 and cumulus cell supplementation in in vitro maturation culture media enhances the viability of human blastocysts

Chatroudi , Khalili MA, Ashourzadeh S, Anbari F, Shahedi A, Safari S

OBJECTIVE: In vitro maturation (IVM) of immature oocytes can be useful for some infertile patients. In IVM programs, the rates of embryo formation and pregnancy are low. Therefore, it is...
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Specific tail swelling pattern in hypo-osmotic solution as a predictor of DNA fragmentation status in human spermatozoa

Kim SW, Nho EJ, Lee JY, Jee BC

OBJECTIVE: The aim of this study was to investigate DNA fragmentation status in human spermatozoa according to specific tail swelling patterns determined via hypo-osmotic swelling test (HOST). METHODS: Frozen semen samples...
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ICSI using fresh and frozen PESA-TESA spermatozoa to examine assisted reproductive outcome retrospectively

Javed A, Ramaiah MK, Talkad MS

OBJECTIVE: The male reproductive system generates, accumulates, and transports the sperm. In this study, 2 methods of surgically retrieving sperm, namely, testicular sperm aspiration (TESA) and percutaneous epididymal sperm aspiration...
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Testicular Fine-Needle Aspiration for Sperm Retrieval in Azoospermia: A Small Step toward the Technical Standardization

Cito G, Coccia ME, Sessa F, Cocci A, Verrienti , Picone R, Fucci R, Criscuoli , Serni S, Carini M, Natali A

PURPOSE: The aim was to describe our preliminary experience performing testicular fine-needle aspiration (TEFNA) with a larger needle in infertile patients with obstructive azoospermia, and to provide a systematic literature...
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What should be done for men with sperm DNA fragmentation?

Kim GY

In an age when a small quantity of sperm can lead to pregnancy through in vitro fertilization or intracytoplasmic sperm injection, selecting healthy sperm is important. Sperm DNA fragmentation (SDF)...
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Clinical assessment of the male fertility

Khatun A, Rahman MS, Pang MG

The evaluation of infertility in males consists of physical examination and semen analyses. Standardized semen analyses depend on the descriptive analysis of sperm motility, morphology, and concentration, with a threshold...
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Successful onco-testicular sperm extraction from a testicular cancer patient with a single testis and azoospermia

Kuroda S, Kondo T, Mori K, Yasuda K, Asai T, Sanjo H, Yakanaka H, Takeshima , Kawahara T, Kato Y, Miyoshi Y, Uemura H, Iwasaki A, Yumura Y

Onco-testicular sperm extraction is used to preserve fertility in patients with bilateral testicular tumors and azoospermia. We report the case of a testicular tumor in the solitary testis of a...
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Developmental competence of in vitro-matured human oocytes obtained from pregnant and non-pregnant women

Pongsuthirak P, Vutyavanich T

OBJECTIVE: The aim of this study was to compare the rate of maturation, fertilization, and embryo development of in vitro-matured human oocytes derived from pregnant and non-pregnant women. METHODS: Immature oocytes...
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ICSI significantly improved the pregnancy rate of patients with a high sperm DNA fragmentation index

Chi HJ, Kim SG, Kim YY, Park JY, Yoo CS, Park IH, Sun HG, Kim JW, Lee KH, Park HD

OBJECTIVE: Correlations between semen parameters and sperm DNA fragmentation index (DFI) were investigated to identify characteristics of sperm without DNA damage that could be used in selecting sperm for intracytoplasmic...
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The association of follicular fluid volume with human oolemma stretchability during intracytoplasmic sperm injection

Inoue T, Yamashita , Tsujimoto , Yamamoto S, Taguchi S, Hirao K, Uemura M, Ikawa K, Miyazaki K

OBJECTIVE: Oocyte degeneration often occurs after intracytoplasmic sperm injection (ICSI), and the risk factor is low-quality oocytes. The follicular fluid (FF) provides a crucial microenvironment for oocyte development. We investigated...
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Comparison of clinical outcomes between in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) in IVF-ICSI split insemination cycles

Lee SH, Lee JH, Park YS, Yang KM, Lim CK

OBJECTIVE: This study aimed to compare the clinical outcomes between in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) in sibling oocytes. Additionally, we evaluated whether the implementation of split...
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Evaluation of human embryo development in in vitro fertilization- and intracytoplasmic sperm injection-fertilized oocytes: A time-lapse study

Kim HJ, Yoon HJ, Jang JM, Lee WD, Yoon SH, Lim JH

OBJECTIVE: We investigated whether the insemination method (in vitro fertilization [IVF] or intracytoplasmic sperm injection [ICSI]) affected morphokinetic events and abnormal cleavage events in embryonic development. METHODS: A total of 1,830...
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Influence of the insemination method on the outcomes of elective blastocyst culture

Wang C, Feng G, Zhang B, Shu J, Zhou H, Gan X, Lin R

OBJECTIVE: The aim of this study was to explore the effects of the insemination method on the outcomes of elective blastocyst culture. METHODS: We retrospectively analyzed the outcomes of elective blastocyst...
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The effects of different types of media on in vitro maturation outcomes of human germinal vesicle oocytes retrieved in intracytoplasmic sperm injection cycles

Fesahat F, Dehghani Firouzabadi R, Faramarzi , Khalili MA

OBJECTIVE: Optimizing in vitro maturation (IVM) media to achieve better outcomes has been a matter of interest in recent years. The aim of this prospective clinical trial was to investigate...
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A successful pregnancy using completely immotile but viable frozen-thawed spermatozoa selected by laser

Chen H, Feng G, Zhang B, Zhou H, Shu J, Gan X

The aim of this study was to report a successful pregnancy using completely immotile frozen-thawed spermatozoa selected by laser. A single laser shot was used to detect the presence of...
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The status of assisted reproductive technology in Korea in 2012

Committee for Assisted Reproductive Technology Statistics, Korean Society for Assisted Reproduction, Lee GH, Song HJ, Choi YM, Han HD

OBJECTIVE: This study was designed to report the status of assisted reproductive technology (ART) therapy in South Korea between January 1, 2012 and December 31, 2012. METHODS: A localized online survey,...
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