- A role of carboxy-terminal region of Toxoplasma gondii-heat shock protein 70 in enhancement of T. gondii infection in mice
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Mun HS, Norose K, Aosai F, Chen M, Yano A
- KMID: 1715245
- Korean J Parasitol.
- 2000 Jun;38(2):107-110.
- doi: 10.3347/kjp.2000.38.2.107
We investigated the role of recombinant Toxoplasma gondii heat shock protein (rT.g.HSP) 70-full length, rT.g.HSP70-NH2-terminal region, or rT.g.HSP70-carboxy-terminal region in prophylactic immunity in C57BL/6 mice perorally infected with Fukaya cysts... -
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- Cleavage of purified neuronal clathrin assembly protein (CALM) by caspase 3 and calpain
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Kim JA, Kim HL
- KMID: 1457537
- Exp Mol Med.
- 2001 Dec;33(4):245-250.
The most efficient means of protein internalization from the membrane are through clathrin-coated pits, which concentrate protein interactions with the clathrin-associated assembly protein complex AP-2 and internalization signals in the... -
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- Cell-free expression and functional reconstitution of CALM in clathrin assembly
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Kim JA, Kim HL
- KMID: 1085745
- Exp Mol Med.
- 2001 Jun;33(2):89-94.
Clathrin-mediated vesicle formation is an essential step in the intracellular trafficking of the protein and lipid. Binding of clathrin assembly protein to clathrin triskelia induces their assembly into clathrin-coated vesicles... -
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- High Expression of Water-Soluble Recombinant Antigenic Domains of Toxoplasma gondii Secretory Organelles
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Yang Z, Ahn HJ, Nam HW
- KMID: 1792197
- Korean J Parasitol.
- 2014 Aug;52(4):367-376.
- doi: 10.3347/kjp.2014.52.4.367
Recombinant antigenic proteins of Toxoplasma gondii are alternative source of antigens which are easily obtainable for serodiagnosis of toxoplasmosis. In this study, highly antigenic secretory organellar proteins, dense granular GRA2... -
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- Synthesis of recombinant blood coagulation factor VIII (FVIII) heavy and light chains and reconstitution of active form of FVIII
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Oh SH, Lee MY, Song DW
- KMID: 1029938
- Exp Mol Med.
- 1999 Jun;31(2):95-100.
FVIII is synthesized as a single chain precursor of approximately 280 kD with the domain structure of A1-A2-B-A3-C1-C2 and it circulates as a series of metal ion-linked heterodimers that result from cleavages... -
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- Diagnostic Efficacy of a Recombinant Cysteine Protease of Spirometra erinacei Larvae for Serodiagnosis of Sparganosis
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Rahman SM, Kim JH, Hong ST, Choi MH
- KMID: 1715669
- Korean J Parasitol.
- 2014 Feb;52(1):41-46.
- doi: 10.3347/kjp.2014.52.1.41
The mature domain of a cysteine protease of Spirometra erinacei plerocercoid larva (i.e., sparganum) was expressed in Escherichia coli, and its value as an antigen for the serodiagnosis of sparganosis... -
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- Gene Cloning, Expression and Immunogenicity of the Protective Antigen Subolesin in Dermacentor silvarum
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Hu Y, Zeng H, Zhang J, Wang D, Li D, Zhang T, Yang S, Liu J
- KMID: 1715679
- Korean J Parasitol.
- 2014 Feb;52(1):93-97.
- doi: 10.3347/kjp.2014.52.1.93
Subolesin (4D8), the ortholog of insect akirins, is a highly conserved protective antigen and thus has the potential for development of a broad-spectrum vaccine against ticks and mosquitoes. To date,... -
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- Localization of Antigenic Sites at the Amino-terminus of Rinderpest Virus N Protein Using Deleted N Mutants and Monoclonal Antibody
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Choi KS, Nah JJ, Ko YJ, Kang SY, Joo YS
- KMID: 1089408
- J Vet Sci.
- 2003 Aug;4(2):167-173.
The nucleocapsid (N) protein of rinderpest virus (RPV) is highly conserved, immunogenic, and abundantly expressed during infection. Six antigenic sites (sites A, B, C, D, E and F), defined previously... -
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- Expression and characterization of the flavoprotein domain of gp91phox
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Han CH, Lee MH
- KMID: 1103470
- J Vet Sci.
- 2000 Jun;1(1):19-26.
Truncated forms of gp91(phox) were expressed in E. coli in which the N-terminal hydrophobic transmembrane region was replaced with a portion of the highly soluble bacterial protein thioredoxin (TRX). TRX-gp91(phox)... -
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- Development of two novel nontoxic mutants of Escherichia coli heat-labile enterotoxin
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Park EJ, Chang JH, Kim JS, Chung SI, Yum JS
- KMID: 1029939
- Exp Mol Med.
- 1999 Jun;31(2):101-107.
Escherichia coli heat-labile enterotoxin (LT) is composed of catalytic A and non-catalytic homo-pentameric B subunits and causes diarrheal disease in human and animals. In order to produce a nontoxic LT for vaccine... -
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- Calcium-influx increases SOD1 aggregates via nitric oxide in cultured motor neurons
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Kim HJ, Im W, Kim S, Kim SH, Sung JJ, Kim M, Lee KW
- KMID: 1090960
- Exp Mol Med.
- 2007 Oct;39(5):574-582.
Familial amyotrophic lateral sclerosis (fALS) is caused by mutations in Cu/Zn-superoxide dismutase (SOD1), and SOD1 aggregation and calcium toxicity are involved in neuronal death. However, the effect of altered calcium... -
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