Korean J Parasitol.  2000 Jun;38(2):107-110. 10.3347/kjp.2000.38.2.107.

A role of carboxy-terminal region of Toxoplasma gondii-heat shock protein 70 in enhancement of T. gondii infection in mice

• Mun HS
• Norose K
• Aosai F
• Chen M
• Yano A

• KMID: 1715245
• DOI: http://doi.org/10.3347/kjp.2000.38.2.107

Abstract

We investigated the role of recombinant Toxoplasma gondii heat shock protein (rT.g.HSP) 70-full length, rT.g.HSP70-NH2-terminal region, or rT.g.HSP70-carboxy-terminal region in prophylactic immunity in C57BL/6 mice perorally infected with Fukaya cysts of T. gondii. At 3, 4, 5, and 6 weeks after infection, the number of T. gondii in the brain tissue of each mouse was measured by quantitative competitive-polymerase chain reaction (QC-PCR) targeting the surface antigen (SAG) 1 gene. Immunization with rT.g.HSP70-full length or r.T.g.HSP70-carboxy-terminal region increased the number of T. gondii in the brain tissue after T. gondii infection, whereas immunization with rT.g.HSP70-NH2-terminal region did not. These results suggest that T.g.HSP70-carboxy-terminal region as well as T.g.HSP70-full length may induce deleterious effects on the protective immunity of mice infected with a cyst-forming T. gondii strain, Fukaya.

MeSH Terms

Animal
Heat-Shock Proteins 70/immunology*
Heat-Shock Proteins 70/genetics
Heat-Shock Proteins 70/chemistry*
Immunization
Mice
Mice, Inbred C57BL
Recombinant Proteins/immunology
Recombinant Proteins/genetics
Recombinant Proteins/chemistry
Toxoplasma/physiology*
Toxoplasmosis, Animal/parasitology*
Toxoplasmosis, Animal/immunology