Korean J Parasitol.  2015 Apr;53(2):237-242. 10.3347/kjp.2015.53.2.237.

Ancient Mitochondrial DNA Analyses of Ascaris Eggs Discovered in Coprolites from Joseon Tomb

Affiliations
  • 1Bioanthropology and Paleopathology Lab, Institute of Forensic Science, Seoul National University College of Medicine, Seoul 110-799, Korea. cuteminjae@gmail.com
  • 2Department of Parasitology and Research Center for Mummy, Dankook University, Cheonan 330-715, Korea.
  • 3Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea.
  • 4Hangang Institute of Cultural Heritage, Seoul 143-904, Korea.
  • 5Sangmyung University, Seoul 110-743, Korea.

Abstract

Analysis of ancient DNA (aDNA) extracted from Ascaris is very important for understanding the phylogenetic lineage of the parasite species. When aDNAs obtained from a Joseon tomb (SN2-19-1) coprolite in which Ascaris eggs were identified were amplified with primers for cytochrome b (cyt b) and 18S small subunit ribosomal RNA (18S rRNA) gene, the outcome exhibited Ascaris specific amplicon bands. By cloning, sequencing, and analysis of the amplified DNA, we obtained information valuable for comprehending genetic lineage of Ascaris prevalent among pre-modern Joseon peoples.

Keyword

Ascaris; ancient DNA; cytochrome b; 18S rRNA; Korean mummy

MeSH Terms

Adult
Animals
Ascariasis/diagnosis/history/*parasitology
Ascaris/classification/genetics/*isolation & purification
Base Sequence
Cytochromes b/genetics
DNA Primers/genetics
DNA, Helminth/*genetics
DNA, Mitochondrial/*genetics/history
Female
Fossils/history/parasitology
History, Ancient
Humans
Male
Molecular Sequence Data
Mummies/history/*parasitology
Ovum/chemistry/classification
Phylogeny
RNA, Ribosomal, 18S/genetics
Cytochromes b
DNA Primers
DNA, Helminth
DNA, Mitochondrial
RNA, Ribosomal, 18S
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