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Comparison of Biotin Interference in Second- and Third-Generation Roche Free Thyroxine Immunoassays

Choi J, Yun SG

No abstract available.
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Urea Synthesis in the Intact and in the Isolated Perfused Liver of the Biotin-Deficient Rats

Kim JH, Cho MY, Kim BW, Song CS

Biotin-deficient rats were raised on a purified ration containing raw egg white plus avidin. Urea synthesis and excretion were compared between the biotin-deficient and the pair-fed control rats. 24hrurinary urea...
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Effect of Avidin and Biotin in Attachment of Human Adipose Stem Cells to Micronized Acellular Dermal Matrix

Yoo G, Rhie JW, Lim JS

  • KMID: 2203165
  • J Korean Soc Plast Reconstr Surg.
  • 2008 Jan;35(1):1-6.
PURPOSE: In tissue engineering, it is important that the scaffolds have high affinity with cells for making efficient use of cells. The authors studied the binding affinity of human adipose...
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Detection of Treponema pallidum in Tissues by the Avidin - Biotin - Peroxidase Complex Techniques

Lee MG, Lee WS, Lee JB

  • KMID: 1680313
  • Korean J Dermatol.
  • 1989 Feb;27(1):23-28.
Using the avidin-biotin-peroxidase complex(ABC) technique, 36 skin specimens from 30 patients with primary and secondary syphilis and a gastric mucosal specimen from a patient with secondary syphilis which were confirmed...
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Immunoradiometric assay of human proinsulin and partially processed proinsulin with use of monoclonal antibody and streptavidin-biotin labeling

Kim JQ, Cho HI, Kim SI, Lee HK, Hales CN

The sensitive and specific immunoradiometric assay is described for human proinsulin and its intermediate peptides (65-66 split and 32-33 split proinsulin). We developed a monoclonal antibody-based two-site immunoradiometric assay with...
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Cultural Characteristics for the Enhanced Mycelial Growth of Ramaria botrytis

Lee TH, Han YH

The culture conditions for the enhanced mycelial growth of Ramaria botrytis was investigated. The optimal temperature and pH for the mycelial growth were 24degrees C and 5.0, respectively. It was...
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Optimization of in situ hybridization assay using non-radioactive DNA probes for the detection of canine herpesvirus (CHV) in paraffin-embedded sections

Kim O

  • KMID: 1094690
  • J Vet Sci.
  • 2004 Mar;5(1):71-73.
Two non-radioactive probes using digoxigenin or biotin were developed for detecting canine herpesvirus (CHV) and compared for their sensitivities by in situ hybridization (ISH) in formalin fixed, paraffin embedded sections,...
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Apoptosis in Rat Thymus after Bolus Intramuscular Injection of 5-Fluorouracil

Kim KH, Sul HJ, Kang DY

  • KMID: 2073714
  • Korean J Pathol.
  • 2000 Jun;34(6):413-418.
We induced apoptosis in normal rats by intramuscular injection of 5-fluorouracil (5-FU) and immunohistochemically evaluated the thymus for the TdT-mediated dUTP biotin nick end labelling on the 1st, 3rd, 6th,...
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A Case of Lennox-Gastaut Syndrome due to 3-Methylcrotonyl CoA Carboxylase Deficiency

Han YS, Kang HC, Lee HJ, Kim HD

  • KMID: 1582226
  • J Korean Child Neurol Soc.
  • 2004 May;12(1):92-98.
3-Methylcrotonyl-CoA carboxylase(MCC) is a biotin-dependent enzyme involved in the leucine metabolism. We describe a patient with MCC deficiency who manifested with Reye syndrome-like illness with status epilepticus, metabolic acidosis,...
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Molecular Links between Caloric Restriction and Sir2/SIRT1 Activation

Wang Y

Ageing is the most significant risk factor for a range of prevalent diseases, including cancer, cardiovascular disease, and diabetes. Accordingly, interventions are needed for delaying or preventing disorders associated with...
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Detection of Treponema pallidum in tissue: a comparative study of the avidin-biotin-peroxidase complex, indirect immunoperoxidase, FTA-ABS complement techniques and the darkfield method

Lee WS, Lee MG, Chung KY, Lee JB

With 37 formalin-fixed, paraffin embedded specimens from the lesions of 30 patients with primary, secondary or gastric syphilis, we performed avidin-biotin-peroxidase complex (ABC), indirect immunoperoxidase (IIP) and FTA-ABS complement techniques....
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Surface interactions between two of the main periodontal pathogens: Porphyromonas gingivalis and Tannerella forsythia

Zhu W, Lee SW

PURPOSE: Porphyromonas gingivalis and Tannerella forsythia have been implicated as the major etiologic agents of periodontal disease. These two bacteria are frequently isolated together from the periodontal lesion, and it...
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Detection of Mycobactrium tuberculosis by in situ hybridization

Park CS, Lee JS, Jung JJ, Kim DH, Kim J

BACKGROUND: A presumptive histopathologic diagnosis of tuberculosis is commonly based on the finding of acid- fast bacilli upon microscopic examination of a diagnostic specimens. Although this traditional histochemical staining methodis...
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Apoptosis of Alveolar Cells in Pneumocystis Carinii Pneumonia: Application of Electron Microscopic Terminal Deoxynucleotidyl Transferase-Mediated dUTP-Biotin Nick End Labeling Method

Kang KH, Kim SP, Kwon KY

  • KMID: 2275589
  • Korean J Pathol.
  • 2001 Dec;35(6):496-505.
BACKGROUND: Pneumocystis carinii (P. carinii) attaches to alveolar cells and causes injury to the epithelial cells by direct toxic effects or inhibition of epithelial growth and replication. Although respiratory cell...
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Changes of the Sialoglycoconjugates in the Maxillary Sinus Mucosa of theRabbits after Inoculation of Influenza a Virus

Cho JH, Jung YG, Cho CS, Park KY, Lim HJ

  • KMID: 1922550
  • Korean J Otolaryngol-Head Neck Surg.
  • 1997 Apr;40(4):600-606.
The present study was carried out in order to observe the changes in the expression of the sialoglycoconjugates of the rabbit sinus mucosa after inoculation of influenza A virus utilizing...
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Affinity Improvement of Antibody-Avidin Fusion Proteins for Biotin

Cho MY, Kim HJ, Cho HM, Shin SU

  • KMID: 1557240
  • Korean J Immunol.
  • 1998 Dec;20(4):381-388.
To generate drug delivery vector to locales in the body, genetic engineering and expression techniques have been used to produce antibody avidin fusion proteins. Chicken avidin has been fused to...
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Apoptotic Keratinocytes in Acrodermatitis Enteropathica

Park YL, Whang KU, Bang SW, Kim YK, Yu HJ, Son SJ

  • KMID: 1891506
  • Korean J Dermatol.
  • 1996 Oct;34(5):753-756.
BACKGROUND: The cause of acrodermatitis enteropathica(AE) is closely related to zinc deficiency. Zinc is a potent inhibitor of endonuclease. Acute rises in the apoptosis in lymphoid and myeloid cell lines...
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Culture Conditions for Mycelial Growth of Coriolus versicolor

Jo WS, Kang MJ, Choi SY, Yoo YB, Seok SJ, Jung HY

Coriolus versicolor, is one of the most popular medicinal mushrooms due its various biologically active components. This study was conducted to obtain basic information regarding the mycelial culture conditions of...
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Effect of seeding using an avidin-biotin binding system on the attachment of periodontal ligament fibroblasts to nanohydroxyapatite scaffolds: three-dimensional culture

Jang YJ, Jung IH, Park JC, Jung UW, Kim CS, Lee YK, Kim CK, Choi SH

  • KMID: 2329629
  • J Periodontal Implant Sci.
  • 2011 Apr;41(2):73-78.
PURPOSE: For periodontal tissue engineering, it is a primary requisite and a challenge to select the optimum types of cells, properties of scaffold, and growth factor combination to reconstruct a...
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Immunohistochemical Expression of Glutathione S-Transferase pai in Hepatocellulae Carcinoma and Cholangiocarcinoma

Choi SR, Han SY, Son YG, Lee JH, Park HS, Roh MH, Choi HJ, Huh GY

  • KMID: 2143755
  • Korean J Gastroenterol.
  • 1998 Jan;31(1):58-63.
BACKGROUND AND AIMS: It has been known that glutathione S-transferase n (GST-n ) is overexpressed in chemically induced precancerous or cancerous lesions of the hepatocyte in animal models. Unlike most...
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