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The production and characterization of anti-Naegleria fowleri monoclonal antibodies

Ryu JS, Im KI

Naegleria fowleri, a free-living amoeba commonly found in moist soil and fresh water, enters the body via the nasal mucosa and migrates along the olfactory nerve to the brain, where...
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Identification of surface antigens of Trichomonas vaginalis

Woo NS, Min DY, Leem MH, Choi YK

Surface proteins of Trichomonas vaginalis (T. vaginalis) were analyzed to study the antigenic variation. The surface proteins of protozoa were labelled by N-hydroxysuccinimide-biotin (NHS-biotin), the NHS-biotin-labelled proteins were immunoprecipitated...
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Immunogenicity and protective efficacy of solubilized merozoite-enriched Theileria sergenti immunogens. I: Protection against homologous stabilate challenge

Baek BK, Choi IH, Kim BS, Hansen R, Kakoma I

Theileria sergenti were isolated from infected erythrocytes by hypotonic lysis, and soluble merozoite antigens were purified by sonication and differential centrifugation. The preparation contained 29, 34, 35 and 105 kD...
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Identification of surface antigen of Trichomonas vaginalis

Min DY, Leem MH, Ahn MH, Ryu JS

Plasma membrane proteins of a Korean isolate of Trichomonas vaginalis HY-1 were fractionated for antigen analysis. Homogenates of T. vaginalis were fractionated by the differential centrifugation using sucrose step-gradient method....
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Comparative antigen analysis of Trichomonas vaginalis by enzyme-linked immunoelectrotransfer blot technique

Min DY, Leem MH, Kim JM, Choi YK

Analysis of six isolates of Trichomonas vaginalis was carried out with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked immunoelectrotransfer blot (EITB). Trichloroacetic acid-treated antigens of the 6 isolates revealed...
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Antigen analysis of Toxoplasma gondii lysate and excretory-secretory materials by enzyme-linked immunoelectrotransfer blot(EITB)

Ahn MH, Son HJ, Leem MH, Min DY

Recently, the importance of toxoplasmosis is raised as a complication in immunosuppressed or AIDS patients. Our study focused on the identification of a variety of Toxoplasma antigens by immunoblotting. Rabbits...
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