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Interaction of Escherichia coli K1 and K5 with Acanthamoeba castellanii Trophozoites and Cysts

Matin A, Jung SY

The existence of symbiotic relationships between Acanthamoeba and a variety of bacteria is well-documented. However, the ability of Acanthamoeba interacting with host bacterial pathogens has gained particular attention. Here, to...
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Evaluation of Cysticidal Effects of Contact Lens Disinfectant on Acanthamoeba Isolates

Lee JE, Park JS, Lee JS, Yoo HS

  • KMID: 2126716
  • J Korean Ophthalmol Soc.
  • 2007 Mar;48(3):431-437.
PURPOSE: To evaluate the cysticidal effect of 5 kinds of commercially available contact lens disinfectants against 2 clinical isolates of Acanthamoeba. METHODS: Five kinds of commercially available contact lens disinfectants were...
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Identification of Protein Arginine Methyltransferase 5 as a Regulator for Encystation of Acanthamoeba

Moon EK, Hong Y, Chung DI, Goo YK, Kong HH

Encystation is an essential process for Acanthamoeba survival under nutrient-limiting conditions and exposure to drugs. The expression of several genes has been observed to increase or decrease during encystation. Epigenetic...
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Efficacy of Korean Multipurpose Contact Lens Disinfecting Solutions against Acanthamoeba castellanii

Moon EK, Park HR, Quan FS, Kong HH

Acanthamoeba keratitis has been increasing in recent years. Main risk factors are contact lens wear and their cleaning solutions. Most contact lens wearers use multipurpose disinfecting solutions (MPDS) for cleansing...
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Identification and Characterization of Protein Arginine Methyltransferase 1 in Acanthamoeba castellanii

Moon EK, Kong HH, Hong Y, Lee HA, Quan FS

Protein arginine methyltransferase (PRMT) is an important epigenetic regulator in eukaryotic cells. During encystation, an essential process for Acanthamoeba survival, the expression of a lot of genes involved in the...
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Short-Cut Pathway to Synthesize Cellulose of Encysting Acanthamoeba

Moon EK, Kong HH

  • KMID: 1792132
  • Korean J Parasitol.
  • 2012 Dec;50(4):361-364.
The mature cyst of Acanthamoeba is highly resistant to various antibiotics and therapeutic agents. Cyst wall of Acanthamoeba are composed of cellulose, acid-resistant proteins, lipids, and unidentified materials. Because cellulose...
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Cytopathic Change and Inflammatory Response of Human Corneal Epithelial Cells Induced by Acanthamoeba castellanii Trophozoites and Cysts

Sohn HJ, Seo GE, Lee JH, Ham AJ, Oh YH, Kang H, Shin HJ

Acanthamoeba castellanii has ubiquitous distribution and causes primary acanthamoebic keratitis (AK). AK is a common disease in contact lens wearers and results in permanent visual impairment or blindness. In this...
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Lethal Effects of Helianthemum lippii (L.) on Acanthamoeba castellanii Cysts in Vitro

Badria FA, Hetta MH, Sarhan RM, Ezz El-Din MH

  • KMID: 1792177
  • Korean J Parasitol.
  • 2014 Jun;52(3):243-249.
Acanthamoeba spp. commonly cause Acanthamoeba keratitis which is typically associated with the wear of contact lenses. Therefore, finding an economic, efficient, and safe therapy of natural origin is of outmost...
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Differentially expressed genes of Acanthamoeba castellanii during encystation

Moon EK, Chung DI, Hong YC, Kong HH

To examine the expressed gene profile during encystation of Acanthamoeba castellanii Castellani, we used differentially expressed gene (DGE) screening by RT-PCR with 20 sets of random primers. From this analysis,...
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The Role of Proteinase in Acanthamoeba keratitis and the Effect of Amniotic Membrane as a Proteinase Inhibitor

Song CH, Song CY, Kim JC

  • KMID: 2205383
  • J Korean Ophthalmol Soc.
  • 1998 Nov;39(11):2527-2541.
This study was performed to investigate the biochemical properties of Acanthamoeba proteinase, its role in the pathogenesis of Acanthamoeba keratitis and the therapeutic effect of the homogenate of amniotic membrane...
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Down-Regulation of Cellulose Synthase Inhibits the Formation of Endocysts in Acanthamoeba

Moon EK, Hong Y, Chung DI, Goo YK, Kong HH

  • KMID: 1792159
  • Korean J Parasitol.
  • 2014 Apr;52(2):131-135.
Acanthamoeba cysts are resistant to unfavorable physiological conditions and various disinfectants. Acanthamoeba cysts have 2 walls containing various sugar moieties, and in particular, one third of the inner wall is...
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DNA Methylation of Gene Expression in Acanthamoeba castellanii Encystation

Moon EK, Hong Y, Lee HA, Quan FS, Kong HH

Encystation mediating cyst specific cysteine proteinase (CSCP) of Acanthamoeba castellanii is expressed remarkably during encystation. However, the molecular mechanism involved in the regulation of CSCP gene expression remains unclear. In...
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Comparison of Proteins Secreted into Extracellular Space of Pathogenic and Non-pathogenic Acanthamoeba castellanii

Moon EK, Choi HS, Park SM, Kong HH, Quan FS

Pathogenic Acanthamoeba spp. cause granulomatous amoebic encephalitis and keratitis. Acanthamoeba keratitis (AK) is a rare but serious ocular infection that can result in permanent visual impairment or blindness. However, pathogenic...
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Molecular and Biochemical Properties of a Cysteine Protease of Acanthamoeba castellanii

Hong Y, Kang JM, Joo SY, Song SM, Lê HG, Thái TL, Lee J, Goo YK, Chung DI, Sohn WM, Na BK

Acanthamoeba spp. are free-living protozoa that are opportunistic pathogens for humans. Cysteine proteases of Acanthamoeba have been partially characterized, but their biochemical and functional properties are not clearly understood yet....
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Effects of Mannose on Pathogenesis of Acanthamoeba castellanii

Yoo KT, Jung SY

  • KMID: 1792133
  • Korean J Parasitol.
  • 2012 Dec;50(4):365-369.
Acanthamoeba spp. are single-celled protozoan organisms that are widely distributed in the environment. In this study, to understand functional roles of a mannose-binding protein (MBP), Acanthamoeba castellanii was treated with...
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Identification of Atg8 Isoform in Encysting Acanthamoeba

Moon EK, Hong Y, Chung DI, Kong HH

  • KMID: 1792139
  • Korean J Parasitol.
  • 2013 Oct;51(5):497-502.
Autophagy-related protein 8 (Atg8) is an essential component of autophagy formation and encystment of cyst-forming parasites, and some protozoa, such as, Acanthamoeba, Entamoeba, and Dictyostelium, have been reported to possess...
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Efficient Liquid Media for Encystation of Pathogenic Free-Living Amoebae

Sohn HJ, Kang H, Seo GE, Kim JH, Jung SY, Shin HJ

Pathogenic Naegleria fowleri, Acanthamoeba castellanii, and Acanthamoeba polyphaga, are distributed worldwide. They are causative agents of primary amoebic meningoencephalitis or acanthamoebic keratitis in humans, respectively. Trophozoites encyst in unfavorable environments,...
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Microarray Analysis of Differentially Expressed Genes between Cysts and Trophozoites of Acanthamoeba castellanii

Moon EK, Xuan YH, Chung DI, Hong Y, Kong HH

Acanthamoeba infection is difficult to treat because of the resistance property of Acanthamoeba cyst against the host immune system, diverse antibiotics, and therapeutic agents. To identify encystation mediating factors of...
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Induction of Anticarcinogenic Enzymes of Waxy Brown Rice Cultured with Phellinus igniarius 26005

Park KB, Ha HC, Kim SY, Kim HJ, Lee JS

  • KMID: 2312385
  • Mycobiology.
  • 2002 Dec;30(4):213-218.
The induction of NAD(P)H: quinone oxidoreductase (QR), glutathione S-transferase (GST), and glutathione (GSH) levels in hepa1c1c7 cells (murine hepatoma) by waxy brown rice cultured with Phellinus igniarius to induce anticarcinogenic...
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Atg3-Mediated Lipidation of Atg8 Is Involved in Encystation of Acanthamoeba

Moon EK, Chung DI, Hong Y, Kong HH

Autophagy is a catabolic process involved in the degradation of a cell's own components for cell growth, development, homeostasis, and the recycling of cellular products. Autophagosome is an essential component...
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