- Monitoring transfected cells without selection agents by using the dual-cassette expression EGFP vectors
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Kang SG, Lee EK, Schaus S, Henderson
- KMID: 938883
- Exp Mol Med.
- 2001 Sep;33(3):174-178.
Conventional methods of selecting gene transfected cells by toxic agents may yield ambiguous results. It is difficult to determine whether cell death is due to selection agents or gene transfection,... -
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- Development of a packaging cell line for propagation of replication-deficient adenovirus vector
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Kim JS, Lee SH, Cho YS, Park KS, Kim YH, Lee JH
- KMID: 938878
- Exp Mol Med.
- 2001 Sep;33(3):145-149.
A human embryonic kidney cell line 293 is widely used for adenovirus production and propagation. With this cell line, however, replication-competent virus (RCV) is frequently generated, especially during large-scale production... -
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- Membrane transducing activity of recombinant Hoxc8 protein and its possible application as a gene delivery vector
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Kim ES, Kang M, Park HW, Kim MH
- KMID: 1052937
- Exp Mol Med.
- 2008 Apr;40(2):151-160.
- doi: 10.3858/emm.2008.40.2.151
In order to examine whether the Hoxc8 protein can deliver nucleic acid into mammalian cells, we designed several Hoxc8-derived recombinant proteins to be synthesized as glutathione S-transferase (GST) fused forms... -
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- Simple Purification of Adeno-Associated Virus-DJ for Liver-Specific Gene Expression
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Liu J, Moon YA
- KMID: 2374103
- Yonsei Med J.
- 2016 May;57(3):790-794.
- doi: 10.3349/ymj.2016.57.3.790
Recombinant gene expression using adeno-associated viruses (AAVs) has become a valuable tool in animal studies, as they mediate safe expression of transduced genes for several months. The liver is a... -
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- Development of an efficient endothelial cell specific vector using promoter and 5' untranslated sequences from the human preproendothelin-1 gene
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Cho JY, Lim WC, Jang S, Lee YJ
- KMID: 1109315
- Exp Mol Med.
- 2003 Aug;35(4):269-274.
We report here, that a vector constructed based on ppET-1 gene promoter and 5' untranslated region induced a high level of gene expression in endothelial cells and the specificity is... -
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