Korean J Physiol Pharmacol.  2011 Oct;15(5):313-317. 10.4196/kjpp.2011.15.5.313.

Intracellular Ca2+ Mobilization and Beta-hexosaminidase Release Are Not Influenced by 60 Hz-electromagnetic Fields (EMF) in RBL 2H3 Cells

Affiliations
  • 1College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea. imss@cau.ac.kr
  • 2Korea EMF Safety, Dankook University, Yongin 448-701, Korea.
  • 3Smart Grid Research Division, Korea Electrotechnology Research Institute, Changwon 641-120, Korea.

Abstract

The effects of extremely low frequency electromagnetic fields (EMF) on intracellular Ca2+ mobilization and cellular function in RBL 2H3 cells were investigated. Exposure to EMF (60 Hz, 0.1 or 1 mT) for 4 or 16 h did not produce any cytotoxic effects in RBL 2H3 cells. Melittin, ionomycin and thapsigargin each dose-dependently increased the intracellular Ca2+ concentration. The increase of intracellular Ca2+ induced by these three agents was not affected by exposure to EMF (60 Hz, 1 mT) for 4 or 16 h in RBL 2H3 cells. To investigate the effect of EMF on exocytosis, we measured beta-hexosaminidase release in RBL 2H3 cells. Basal release of beta-hexosaminidase was 12.3+/-2.3% in RBL 2H3 cells. Exposure to EMF (60 Hz, 0.1 or 1 mT) for 4 or 16 h did not affect the basal or 1 microM melittin-induced beta-hexosaminidase release in RBL 2H3 cells. This study suggests that exposure to EMF (60 Hz, 0.1 or 1 mT), which is the limit of occupational exposure, has no influence on intracellular Ca2+ mobilization and cellular function in RBL 2H3 cells.

Keyword

EMF; Ca2+ mobilization; Exocytosis; Beta-hexosaminidase

MeSH Terms

beta-N-Acetylhexosaminidases
Electromagnetic Fields
Exocytosis
Ionomycin
Melitten
Occupational Exposure
Thapsigargin
Ionomycin
Melitten
Thapsigargin
beta-N-Acetylhexosaminidases

Figure

  • Fig. 1. The effect of EMF on cell viability of RBL 2H3 cells. The cells were exposed to EMF (60 Hz, 0.1 or 1 mT) for 4 or 16 h and viability was measured with MTT assay. Results are indicated in mean±S.D. from four separate experiments.

  • Fig. 2. The effect of EMF on the intracellular Ca2+ mobilization induced by melittin in RBL 2H3 cells. Intracellular Ca2+ mobilization induced by melittin was measured with Quanta Master in Fura-2AM-loaded RBL 2H3 cells. Melittin dose-dependently increased intracellular Ca2+ mobilization (A) and 0.5μM melittin-induced intracellular Ca2+ mobilization was not affected by EMF (60 Hz, 1 mT) for 4 or 16 h (B). Results are the representative data of four separate experiments.

  • Fig. 3. The effect of EMF on intracellular Ca2+ mobilization induced by ionomycin in RBL 2H3 cells. Ionomycin dose-dependently increased intracellular Ca2+ mobilization (A) and 10 nM ionomycin-induced intracellular Ca2+ mobilization was not affected by EMF (60 Hz, 1 mT) for 4 or 16 h (B). Results are the representative data of four separate experiments.

  • Fig. 4. The effect of EMF on intracellular Ca2+ mobilization induced by thapsigargin in RBL 2H3 cells. Thapsigargin dose-dependently increased intracellular Ca2+ mobilization (A) and 100 nM thapsigargin-induced intracellular Ca2+ mobilization was not affected by EMF (60 Hz, 1 mT) for 4 or 16 h (B). Results are the representative data of four separate experiments.

  • Fig. 5. The effect of EMF on basal (A) and 1μM melittin-induced beta-hexosaminidase release (B) in RBL 2H3 cells. Both basal and 1μM melittin-induced beta-hexosaminidase release were not affected by exposure to EMF (60 Hz, 0.1 or 1 mT) for 4 or 16 h. Results indicate mean±S.D. from four separate experiments.


Reference

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