- Rapid Prenatal Detection of Down and Edwards Syndromes by Fluorescent Polymerase Chain Reaction with Short Tandem Repeat Markers
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Yoon HR, Park YS, Kim YK
- KMID: 1728847
- Yonsei Med J.
- 2002 Oct;43(5):557-566.
- doi: 10.3349/ymj.2002.43.5.557
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- Detection of Bartonella henselae DNA by Polymerase Chain Reaction in a Patient with Cat Scratch Disease: A Case Report
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Chung JY, Han TH, Kim BN, Yoo YS, Lim SJ
- KMID: 1781778
- J Korean Med Sci.
- 2005 Oct;20(5):888-891.
- doi: 10.3346/jkms.2005.20.5.888
We report a case of cat scratch disease caused by Bartonella henselae in Korea. A 25-yr-old woman developed left cervical lymphadenopathy with history of contact with a dog. The cervical... -
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- Comparative Analysis of Polymerase Chain Reaction Assay for Herpes Simplex Virus 1 Detection in Tear
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Lee SY, Kim MJ, Kim MK, Wee WR
- KMID: 1707284
- Korean J Ophthalmol.
- 2013 Oct;27(5):316-321.
- doi: 10.3341/kjo.2013.27.5.316
PURPOSE: To comparatively analyze the methodological efficacy of the polymerase chain reaction (PCR) assay for herpes simplex virus 1 (HSV) detection in tears. METHODS: This retrospective study reviewed the medical records... -
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- Development of a Lightcycler-based reverse transcription polymerase chain reaction for the detection of foot-and-mouth disease virus
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Oem JK, Kye SJ, Lee KN, Kim YJ, Park JY, Park JH, Joo YS, Song HJ
- KMID: 1091201
- J Vet Sci.
- 2005 Sep;6(3):207-212.
One step TaqMan real-time reverse transcription polymerase chain reaction (R/T RT-PCR)using a set of primers/probes was developed for the detection of foot-and-mouth disease (FMD)virus. The gene-specific probes labeled fluorogen for... -
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- Alibi Verification and the PCR Method to Estimate the Source of Epidemic for a Few Notified Cases of S. sonnei
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Lee SJ, Koh DH, Yoon CH
- KMID: 792372
- J Prev Med Public Health.
- 2005 Nov;38(4):420-424.
OBJECTIVES: A few culture-confirmed cases of S. sonnei have been notified from Korean hospitals. The source of epidemic can't be firmly determined in such cases because of the rarity of... -
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- Comparison of In-house PCR with Conventional Techniques and Cobas Amplicor M. tuberculosis(TM) Kit for Detection of Mycobacterium tuberculosis
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Kim MH, Yang HY, Suh JT, Lee HJ
- KMID: 1793188
- Yonsei Med J.
- 2008 Aug;49(4):537-544.
- doi: 10.3349/ymj.2008.49.4.537
PURPOSE: Polymerase chain reaction (PCR) assay, introduced as a fast and sensitive diagnostic method, is useful in detecting Mycobacterium tuberculosis. The purpose of this study was to evaluate the usefulness... -
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- A Melting Method for RNA Extraction from the Mucosal Membrane of the Mouse Middle Ear
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Seo YJ, Kim SH, Moon IS, Choi JY
- KMID: 2070030
- Yonsei Med J.
- 2015 Mar;56(2):497-502.
- doi: 10.3349/ymj.2015.56.2.497
PURPOSE: There is much confusion surrounding the methods of RNA extraction from the middle ear mucosa of mice. In this study, we worked to develop a "melting method," which is... -
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- Multiplex PCR for the Detection of Genes Encoding Aminoglycoside Modifying Enzymes and Methicillin Resistance among Staphylococcus Species
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Choi SM, Kim SH, Kim HJ, Lee DG, Choi JH, Yoo JH, Kang JH, Shin WS, Kang MW
- KMID: 1130030
- J Korean Med Sci.
- 2003 Oct;18(5):631-636.
- doi: 10.3346/jkms.2003.18.5.631
We developed multiplex polymerase chain reaction (PCR) to detect aac(6 ')/aph(2 "), aph(3 ')-IIIa, and ant(4 ')-Ia, the genes encoding the most clinically relevant amino-glycoside modifying enzymes (AME), and simultaneously,... -
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- Detection of canine distemper virus (CDV) through one step RT-PCR combined with nested PCR
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Kim YH, Cho KW, Youn HY, Yoo HS, Han HR
- KMID: 1103508
- J Vet Sci.
- 2001 Apr;2(1):59-63.
A one step reverse transcription PCR (RT-PCR) combined nested PCR was set up to increase efficiency in the diagnosis of canine distemper virus (CDV) infection after developement of nested PCR.... -
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- Simultaneous detection of Lawsonia intracellularis, Brachyspira hyodysenteriae and Salmonella spp. in swine intestinal specimens by multiplex polymerase chain reaction
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Suh DK, Song JC
- KMID: 1091205
- J Vet Sci.
- 2005 Sep;6(3):231-237.
A multiplex PCR assay was developed for the simultaneous detection of the etiologic agents associated with porcine proliferative enteropathies (PPE), swine dysentery (SD)and porcine salmonellosis (PS)in a single reaction using... -
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- Identification of Adenovirus, Influenza Virus, Parainfluenza Virus, and Respiratory Syncytial Virus by Two Kinds of Multiplex Polymerase Chain Reaction (PCR) and a Shell Vial Culture in Pediatric Patients with Viral Pneumonia
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Lee JH, Chun JK, Kim DS, Park Y, Choi JR, Kim HS
- KMID: 1071430
- Yonsei Med J.
- 2010 Sep;51(5):761-767.
- doi: 10.3349/ymj.2010.51.5.761
PURPOSE: Early identification of causative agents in lower respiratory infection of pediatric patients can reduce morbidity and prevent an overuse of antimicrobials. Two kinds of multiplex polymerase chain reaction (PCR)... -
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- Rapid ABO Genotyping Using Whole Blood without DNA Purification
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Lee SH, Park G, Yang YG, Lee SG, Kim SW
- KMID: 1096975
- Korean J Lab Med.
- 2009 Jun;29(3):231-237.
- doi: 10.3343/kjlm.2009.29.3.231
BACKGROUND: ABO genotyping is commonly used in cases of an ABO discrepancy between cell typing and serum typing, as well as in forensic practice for personal identification and paternity testing.... -
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- Comparative Evaluation of Several Gene Targets for Designing a Multiplex-PCR for an Early Diagnosis of Extrapulmonary Tuberculosis
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Raj A, Singh N, Gupta KB, Chaudhary , Yadav A, Chaudhary , Agarwal K, Varma-Basil M, Prasad R, Khuller GK, Mehta PK
- KMID: 2466356
- Yonsei Med J.
- 2016 Jan;57(1):88-96.
- doi: 10.3349/ymj.2016.57.1.88
PURPOSE: Diagnosis of extrapulmonary tuberculosis (EPTB) poses serious challenges. A careful selection of appropriate gene targets is essential for designing a multiplex-polymerase chain reaction (M-PCR) assay. MATERIALS AND METHODS: We compared... -
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- Detection of Mycobacterium tuberculosis complex Using Real-time Polymerase Chain Reaction
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Chang HE, Heo SR, Yoo KC, Song SH, Kim SH, Kim HB, Park KU, Song J, Lee JH, Park SS, Kim EC
- KMID: 854836
- Korean J Lab Med.
- 2008 Apr;28(2):103-108.
- doi: 10.3343/kjlm.2008.28.2.103
BACKGROUND: For the detection of Mycobacterium tuberculosis complex (MTB), PCR is known to be sensitive, specific, and rapid compared to the conventional methods of acid-fast-bacilli (AFB) smear and culture.... -
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- Evaluation of BioSewoom(TM) HLA-A, -B, -C PCR/SSP Kit
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Cha CH, Kim MH, Chung HJ, Choi SE, Oh HB
- KMID: 1781536
- Korean J Lab Med.
- 2007 Oct;27(5):360-368.
- doi: 10.3343/kjlm.2007.27.5.360
BACKGROUND: Human leukocyte antigen (HLA) typing based on polymerase chain reaction (PCR) is rapidly replacing the conventional serological method. This study was intended to evaluate Bio- SewoomTM HLA-A, -B,... -
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- Detection of 12 Respiratory Viruses with Two-set Multiplex Reverse Transcriptase-PCR Assay Using a Dual Priming Oligonucleotide System
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Yoo SJ, Kuak EY, Shin BM
- KMID: 1781546
- Korean J Lab Med.
- 2007 Dec;27(6):420-427.
- doi: 10.3343/kjlm.2007.27.6.420
BACKGROUND: We intended to evaluate the diagnostic usefulness of a multiplex reverse transcriptase- PCR (mRT-PCR) assay kit under dual priming oligonucleotide system (DPO) for the childhood acute respiratory tract... -
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- Search for Genes Potentially Related to Germ Tube Formation in Candida albicans by Differential-Display Reverse Transcription Polymerase Chain Reaction
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Park JY, Kim DH, Shin WS, Seo HS, Kim KH, Lee KH, Koh CM, Bong JP
- KMID: 1719077
- Yonsei Med J.
- 2003 Feb;44(1):110-118.
- doi: 10.3349/ymj.2003.44.1.110
Candida albicans exhibits the ability to grow in either a yeast or a mycelia form in response to different environmental factors. The mycelia form, found in infected tissues, is important... -
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- Identification of a Rare 3 bp BRAF Gene Deletion in a Thyroid Nodule by Mutant Enrichment with 3'-Modified Oligonucleotides Polymerase Chain Reaction
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Jang MA, Lee ST, Oh YL, Kim SW, Chung JH, Ki CS, Kim JW
- KMID: 1381693
- Ann Lab Med.
- 2012 May;32(3):238-241.
- doi: 10.3343/alm.2012.32.3.238
Papillary thyroid carcinoma (PTC) is the most common malignant thyroid tumor, and 36-69% of PTC cases are caused by mutations in the BRAF gene. The substitution of a valine for... -
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- Comparison of the Urine ThinPrep Test and Melanoma Antigen Gene RT-PCR for the Detection of Bladder Cancer According to Three Urine Collection Methods
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Kim DY, Cho HJ, Oh HK, Shin IH, Jeon CH
- KMID: 1121660
- Korean J Lab Med.
- 2007 Feb;27(1):50-55.
- doi: 10.3343/kjlm.2007.27.1.50
BACKGROUND: For the detection of transitional cell carcinoma (TCC) of the bladder, we compared the sensitivities and specificities between the ThinPrep test and Melanoma Antigen Gene (MAGE) test with... -
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- Diagnostic Usefulness of Genomic Breakpoint Analysis of Various Gene Rearrangements in Acute Leukemias: A Perspective of Long Distance- or Long Distance Inverse-PCR-based Approaches
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Yang JJ, Marschalek R, Meyer C, Park TS
- KMID: 1380095
- Ann Lab Med.
- 2012 Jul;32(4):316-318.
- doi: 10.3343/alm.2012.32.4.316
No abstract available. -
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