Korean J Ophthalmol.  2000 Jun;14(1):27-31. 10.3341/kjo.2000.14.1.27.

Effect of IOP elevation on matrix metalloproteinase-2 in rabbit anterior chamber

• Kim CY
• Kim SS
• Koh HJ
• You YS
• Seong GJ
• Hong YJ

• KMID: 754269
• DOI: http://doi.org/10.3341/kjo.2000.14.1.27

Abstract

To investigate changes in the level of matrix metalloproteinase-2 (MMP-2) in the anterior chamber of rabbit with intraocular pressure (IOP) elevation. The IOP was elevated with scleral encircling in 12 rabbits. In the control group (4 rabbits), IOP was not changed after scleral encircling, and in group 1 (4 rabbits) and 2 (4 rabbits), IOP was elevated about 10 and 20 mmHg respectively after scleral encircling. At 2 days after scleral encircling, aqueous sampling was performed and levels of MMP-2 were checked by Western blots and gelatin zymograms. The greater the IOP elevation, the more MMP-2 expression in the anterior chamber by Western blots and gelatin zymograms. The increase in MMP-2 expression in response to IOP elevation may have important implications for the IOP feedback control mechanism.

MeSH Terms

Animal
Anterior Chamber/enzymology+ACo-
Aqueous Humor/enzymology+ACo-
Biological Markers
Blotting, Western
Comparative Study
Gelatinase A/metabolism+ACo-
Intraocular Pressure+ACo-
Ocular Hypertension/etiology
Ocular Hypertension/enzymology+ACo-
Rabbits
Sclera/surgery
Surgical Sponges/adverse effects