Ann Lab Med.  2023 May;43(3):310-314. 10.3343/alm.2023.43.3.310.

The First Case of Acute Myeloid Leukemia With t(10;11)(p13;q21);PICALM-MLLT10 Rearrangement Presenting With Extensive Skin Involvement

• Park MS
• Kim HY
• Lee JJ
• Cho D
• Jung CW
• Kim HJ
• Kim SH

• KMID: 2551718
• DOI: http://doi.org/10.3343/alm.2023.43.3.310

Figure

• 

  Fig. 1 Bone marrow (BM) aspiration and biopsy. Medium-to-large size leukemic blasts were observed in (A) BM aspiration (Wright-Giemsa stain, ×400) and (B) biopsy (hematoxylin and eosin stain, ×100). In leukemic blasts, (C) myeloperoxidase (MPO) were positive, (D) CD3 were negative, (E) CD34 were positive, and (F) terminal deoxynucleotidyl transferase (TdT) immunostains (×100) were partially positive. (G-J) Skin biopsy of trunk. (G) Leukemic blasts were observed (hematoxylin and eosin stain, ×100); (H) MPO was positive (×400) and (I) CD3 and (J) CD20 were negative (×100).

• 

  Fig. 2 Cytogenetic and molecular analysis of bone marrow study (A) Chromosomal analysis showed 46,XY,t(10;11)(p13;q21),del(17)(p12). Each arrow indicates the reciprocal translocation between 10p13 and 11q21, and deletion of 17p. (B) The PICAM-MLLT10 fusion transcript was identified via agarose gel electrophoresis (Lane 1, internal control using GAPDH [amplicon size, 131 bp]; lanes 2 and 3, PICALM-MLLT10 fusion transcripts identified using the PICALM exon 19 and MLLT10 exon 7 primer pair 1 [358 bp] and PICALM exon 19 and MLLT10 exon 11 primer pair 2 [922 bp], respectively) and Sanger sequencing. Chromatogram of Sanger sequencing shows the PICALM exon 19-MLLT10 exon 3 fusion.

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