J Yeungnam Med Sci.  2023 Nov;40(Suppl):S9-S16. 10.12701/jyms.2023.00150.

Analysis of single nucleotide polymorphisms associated with the vitamin D pathway in the placentas of women with gestational diabetes mellitus: a laboratory study

• Kim MJ
• Kim HM
• Cha HH
• Seong WJ

• KMID: 2548337
• DOI: http://doi.org/10.12701/jyms.2023.00150

Abstract

Background
The aim of this study was to analyze the single nucleotide polymorphisms (SNPs) of genes known to be involved in vitamin D metabolism in the placenta using the placental tissue of mothers diagnosed with gestational diabetes mellitus (GDM) to determine whether the SNPs and occurrence of GDM are related.
Methods
We enrolled 80 women of the same gestational age, 40 with and 40 without GDM. The placenta was obtained from each woman after delivery and SNP genotyping was performed on seven SNPs in the CYP27B1 (rs10877012), CYP24A1 (rs2248359, rs6013897, and rs2209314), and GC (rs2282679, rs16847024, and rs3733359) genes. Maternal serum 25-hydroxyvitamin D levels were measured during the first trimester of pregnancy and before delivery.
Results
At the time of delivery, vitamin D levels were lower (21.05±12.05 mg/dL vs. 31.31±20.72 mg/dL, p=0.012) and the frequency of vitamin D deficiency was higher (60.7% vs. 32.5%, p=0.040) in the GDM group. In women with GDM, the G allele of rs10877012 was more common (86.3% vs. 65.0%, p=0.002). The rs10877012 GG genotype was more common in the GDM group (72.5% vs. 42.5%, p=0.007) and the rs10877012 TT genotype was more common in the control group (12.5% vs. 0%, p=0.007).
Conclusion
Mothers with GDM have lower serum concentrations of vitamin D before delivery than healthy controls and vitamin D deficiency is common. A polymorphism in CYP27B1 (rs10877012), is considered to be a cause of GDM pathogenesis.

Keyword

Gestational diabetes mellitus; rs10877012; Single nucleotide polymorphism; Vitamin D deficiency

Figure

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  Fig. 1. Patient flow diagram in this study. All women deliver at full term with no fetal chromosomal or structural anomalies. If a woman has pregnancy-related complications and risks, such as multiple pregnancies, gestational hypertension, preeclampsia, preterm labor, or fetal growth restrictions, she is excluded from the study. VITD, vitamin D; GDM, gestational diabetes mellitus; IUGR, intrauterine growth restriction.

Reference

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