Restor Dent Endod.  2023 Feb;48(1):e1. 10.5395/rde.2023.48.e1.

Dentinal tubule penetration of sodium hypochlorite in root canals with and without mechanical preparation and different irrigant activation methods

• Oliveira RAd
• Weissheimer T
• Só GB
• Rosa RAd
• Souza MA
• Ribeiro RG
• Só MVR

• KMID: 2548169
• DOI: http://doi.org/10.5395/rde.2023.48.e1

Abstract

Objectives
This study evaluated the dentinal penetration depth of 2.5% sodium hypochlorite (NaOCl) in root canals with and without preparation and different irrigant activation protocols.
Materials and Methods
Sixty-three bovine mandibular incisors were randomly allocated to 6 groups (n = 10): G1, preparation + conventional needle irrigation (CNI); G2, preparation + passive ultrasonic irrigation (PUI); G3, preparation + Odous Clean (OC); G4, no preparation + CNI; G5, no preparation + PUI; G6, no preparation + OC; and CG (negative control; n = 3). Samples were filled with crystal violet for 72 hours. Irrigant activation was performed. Samples were sectioned perpendicularly along the long axis, 3 mm and 7 mm from the apex. Images of the root thirds of each block were captured with a stereomicroscope and analyzed with an image analysis software. One-way analysis of variance, followed by the Tukey post hoc test, and the Student’s t-test were used for data analysis, with a significance level of 5%.
Results
The NaOCl penetration depth was similar when preparation was performed, regardless of the method of irrigation activation (p > 0.05). In the groups without preparation, G6 showed greater NaOCl penetration depth (p < 0.05). The groups without preparation had a greater NaOCl penetration depth than those with preparation (p = 0.0019).
Conclusions
The NaOCl penetration depth was similar in groups with root canal preparation. Without root canal preparation, OC allowed deeper NaOCl penetration. The groups without preparation had greater NaOCl penetration than those undergoing root canal preparation.

Keyword

Dentin penetration; Endodontic; Regenerative endodontic; Smear layer; Sodium hypochlorite

Figure

• 

  Figure 1 Representative image, using the ImageJ program, showing the measurement of the bleached area promoted by sodium hypochlorite.

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  Figure 2 Representative images of the dentinal penetration of sodium hypochlorite in samples from the middle third from groups with root canal preparation (G1-G3: A-C, respectively), without root canal preparation (G4-G6: D-F, respectively), and the GC (G-I).

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  Figure 3 Representative image showing greater penetration of sodium hypochlorite in the cervical third than in the apical third in the G6 group. Arrows indicate the bleached areas.

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