Restor Dent Endod.  2021 Nov;46(4):e58. 10.5395/rde.2021.46.e58.

The effect of using nanoparticles in bioactive glass on its antimicrobial properties

• Obeid MF
• El-Batouty KM
• Aslam M

• KMID: 2548102
• DOI: http://doi.org/10.5395/rde.2021.46.e58

Abstract

Objectives
This study addresses the effect of using nanoparticles (np) on the antimicrobial properties of bioactive glass (BAG) when used in intracanal medicaments against Enterococcus faecalis (E. faecalis) biofilms.
Materials and Methods
E. faecalis biofilms, grown inside 90 root canals for 21 days, were randomly divided into 4 groups according to the antimicrobial regimen followed (n = 20; BAG-np, BAG, calcium hydroxide [CaOH], and saline). After 1 week, residual live bacteria were quantified in terms of colony-forming units (CFU), while dead bacteria were assessed with a confocal laser scanning microscope.
Results
Although there was a statistically significant decrease in the mean CFU value among all groups, the nano-group performed the best. The highest percentage of dead bacteria was detected in the BAG-np group, with a significant difference from the BAG group.
Conclusions
The reduction of particle size and use of a nano-form of BAG improved the antimicrobial properties of the intracanal treatment of E. faecalis biofilms

Keyword

Bioactive glass; Biofilm; Confocal laser scanning microscopy; Nanoparticles

Figure

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  Figure 1 Transmission electron microscopy scan showing the particle size of the bioactive glass nanoparticles.

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  Figure 2 Scanning electron microscope images showing Enterococcus faecalis biofilm on (A) dentin surface and in (B) dentinal tubules after 21 days.

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  Figure 3 Confocal laser scan images of 1: bioactive glass nanoparticles, 2: bioactive glass, 3: calcium hydroxide, and 4: saline. The green fluorescence represents live bacteria, while the red fluorescence represents dead bacteria. (A) fluorescence of live bacteria, (B) fluorescence of dead bacteria, (C) A+B.

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