J Gynecol Oncol.  2022 Aug;33(S1):S4. 10.3802/jgo.2022.33.S4.

Single-cell transcriptomics reveals tissue architecture in ovarian carcinosarcoma

• Xu J
• Lu W

• KMID: 2537925
• DOI: http://doi.org/10.3802/jgo.2022.33.S4

Abstract

Objective
Ovarian carcinosarcoma (OCS) is one of rarest and most challenging histologic subtype of ovarian cancer. It features remarkable cellular heterogeneity. Using single-cell RNA sequencing (scRNA-seq), we characterize the cellular composition of OCS and identify their molecular characteristics.
Methods
We applied scRNA-seq to resected primary OCS for the in-depth analysis of tumor cells and the tumor microenvironment. Immunohistochemistry staining was used for validation.
Results
Malignant epithelial and fibroblast cells displayed a high-degree of intratumoral heterogeneity. We revealed that certain epithelial cell subclusters had high levels of drug resistance scores and many active metabolic pathways. Furthermore, γδ T cells exhibited enriched interferon (IFN)-γ and IFN-α response characteristics. Analyzing ligand-receptor interaction pairs between cell types, we identified broadly interacting cells and observed an interaction between the ANXA1+ epithelial population and FPR1+/FPR3+ myeloid cells.
Conclusion
Our findings provide a single-cell transcriptomic signature of human OCS and present a well-established resource for elucidating OCS diversity.