Mycobiology.  2021 Aug;49(4):376-384. 10.1080/12298093.2021.1940746.

Evaluation of Genetic Diversity and Population Structure Analysis among Germplasm of Agaricus bisporus by SSR Markers

  • 1Department of Industrial Plant Science and Technology, Chungbuk National University, Cheongju, Republic of Korea
  • 2Department of Forest Science, Chungbuk National University, Cheongju, Republic of Korea
  • 3Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Eumseong, Republic of Korea
  • 4Department of Crop Science, Chungbuk National University, Cheongju, Republic of Korea


Agaricus bisporus is a popular edible mushroom that is cultivated worldwide. Due to its secondary homothallic nature, cultivated A. bisporus strains have low genetic diversity, and breeding novel strains is challenging. The aim of this study was to investigate the genetic diversity and population structure of globally collected A. bisporus strains using simple sequence repeat (SSR) markers. Agaricus bisporus strains were divided based on genetic distance-based groups and model-based subpopulations. The major allele frequency (MAF), number of genotypes (NG), number of alleles (NA), observed heterozygosity (HO), expected heterozygosity (HE), and polymorphic information content (PIC) were calculated, and genetic distance, population structure, genetic differentiation, and Hardy–Weinberg equilibrium (HWE) were assessed. Strains were divided into two groups by distance-based analysis and into three subpopulations by model-based analysis. Strains in subpopulations POP A and POP B were included in Group I, and strains in subpopulation POP C were included in Group II. Genetic differentiation between strains was 99%. Marker AB-gSSR-1057 in Group II and subpopulation POP C was confirmed to be in HWE. These results will enhance A. bisporus breeding programs and support the protection of genetic resources.


Agaricus bisporus; genetic diversity; population structure
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