J Pathol Transl Med.  2021 Jul;55(4):279-288. 10.4132/jptm.2021.05.10.

Correlation of TTF-1 immunoexpression and EGFR mutation spectrum in non–small cell lung carcinoma

Affiliations
  • 1Department of Pathology, All India Institute of Medical Sciences, New Delhi, India
  • 2Department of Medical Oncology, Dr B.R. Ambedkar Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi, India
  • 3Department of Pulmonary Medicine and Sleep Disorders, All India Institute of Medical Sciences, New Delhi, India

Abstract

Background
Thyroid transcription factor (TTF-1) is a diagnostic marker expressed in 75%–85% of primary lung adenocarcinomas (ACs). Activating mutations in the tyrosine kinase domain of the epidermal growth factor receptor (EGFR) gene is the most common targetable driver alteration in lung AC. Previous studies have shown a positive correlation between TTF-1 and EGFR mutation status. We aimed to determine the predictive value of TTF-1 immunoexpression for underlying EGFR mutation status in a large Indian cohort.
Methods
This retrospective designed study was conducted with medical record data from 2011 to 2020. All cases of primary lung AC and non–small cell lung carcinoma not otherwise specified (NSCLC, NOS) with known TTF-1 expression diagnosed by immunohistochemistry using 8G7G3/1 antibodies and EGFR mutation status diagnosed by quantitative polymerase chain reaction were retrieved, reviewed, and the
results
were analyzed. Results: Among 909 patient samples diagnosed as lung AC and NSCLC, NOS, TTF-1 was positive in 76.8% cases (698/909) and EGFR mutations were detected in 29.6% (269/909). A strong positive correlation was present between TTF-1 positivity and EGFR mutation status (odds ratio, 3.61; p < .001), with TTF-1 positivity showing high sensitivity (90%) and negative predictive value (87%) for EGFR mutation. TTF-1 immunoexpression did not show significant correlation with uncommon/dual EGFR mutations (odds ratio, 1.69; p = .098). EGFR–tyrosine kinase inhibitor therapy was significantly superior to chemotherapy among EGFR mutant cases irrespective of TTF-1 status; however, no significant differences among survival outcomes were observed.
Conclusions
Our study confirms a strong positive correlation between TTF-1 expression and common EGFR mutations (exon 19 deletion and exon 21 L858R) in advanced lung AC with significantly high negative predictive value of TTF-1 for EGFR mutations.

Keyword

EGFR mutation; Lung adenocarcinoma; Non–small cell lung carcinoma; Thyroid transcription factor-1; Uncommon mutations

Figure

  • Fig. 1. Photomicrographs of non–small cell lung carcinoma (NSCLC). Epidermal growth factor receptor (EGFR) mutant lung adenocarcinoma with acinar and solid patterns (A) showing nuclear staining for thyroid transcription factor 1 (TTF-1) in tumor cells (B). EGFR wild type NSCLC with solid architecture (C) immunopositive for TTF-1 (D). Examples of EGFR mutant NSCLC not otherwise specified (NOS) (E) and EGFR wild type NSCLC, NOS (G) that are negative for TTF-1 (F, H); EGFR mutant invasive mucinous adenocarcinoma (I).

  • Fig. 2. Forest plot for correlation between thyroid transcription factor 1 and epidermal growth factor receptor (EGFR) mutation status in clinicopathological subgroups. Subgroup-specific odds ratios (ORs) are denoted by black dots (line width corresponding to 95% confidence intervals). Combined OR estimate for all patients is represented by a black diamond, where the width corresponds to 95% confidence intervals.

  • Fig. 3. Bar diagram showing histopathological subtyping of four groups based on thyroid transcription factor 1 (TTF-1) and epidermal growth factor receptor (EGFR) mutation status (A) and spectrum of EGFR mutations (B) in these groups. AC, adenocarcinoma; IMA, invasive mucinous adenocarcinoma; LCC, large cell carcinoma; NSCLC, non small cell lung carcinoma; NOS, not otherwise specified.


Reference

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