Korean J Blood Transfus.  2019 Aug;30(2):113-123. 10.17945/kjbt.2019.30.2.113.

Identification of MicroRNA Related to the CD34+ Cell Fraction of Cord Blood Stem Cells

Affiliations
  • 1Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea. jeannie@snu.ac.kr
  • 2Department of Laboratory Medicine, SMG-SNU Boramae Medical Center, Seoul, Korea.
  • 3Seoul Metropolitan Government Public Cord Blood Bank (Allcord), Seoul, Korea.

Abstract

BACKGROUND
Cord blood (CB) is a reliable source of hematopoietic stem cells, and its utilization in stem cell transplantation is increasing continuously. The CD34+ cell count is arguably one of the most important parameters for evaluating the quality of a cord blood unit (CBU), but there is little evidence on the post-genetic modifications that can affect the CD34+ cell counts. In this study, the difference in the miRNA expression profiles between low and high CD34+ CBU was evaluated.
METHODS
Paired CB and maternal samples with low (<0.06%) and high CD34+ cell counts (>0.9%) were selected for analysis. MicroRNA profiling was performed, and differentially expressed miRNA were identified. In addition, gene ontology analysis was conducted on the miRNA to elucidate the genes that could potentially affect the CD34+ cell count.
RESULTS
Ten miRNA were identified to show significantly different expression between the low and high CD34+ groups. Four of the 10 miRNA were hematopoiesis-related (miR-199a-5p, miR-22-5p, miR-140-5p, and miR-181b-5p). From a total of 119 associated genes, nine (CALCA, FARP2, FSHR, ITGAM, MELK, MLF1, PRG4, TREM2 and VCAM1) were associated with two or more of the aforementioned miRNA.
CONCLUSION
This is the first study that examined the difference in the miRNA expression profiles between high and low CD34+ CB cells and revealed the relevant genes associated with hematopoiesis. These results provide basic insight into the genetic processes involving hematopoietic stem cell proliferation.

Keyword

MicroRNA; CD34; Cord blood; Stem cell transplantation
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