J Pathol Transl Med.  2018 Sep;52(5):307-313. 10.4132/jptm.2018.08.03.

Interleukin-31, Interleukin-31RA, and OSMR Expression Levels in Post-burn Hypertrophic Scars

Affiliations
  • 1Department of Pathology, Hallym University College of Medicine, Chuncheon, Korea.
  • 2Department of Pathology, Hallym University Dongtan Sacred Heart Hospital, Hwaseong, Korea.
  • 3Department of Anesthesiology and Pain Medicine, Hallym University Hangang Sacred Heart Hospital, Seoul, Korea.
  • 4Department of Pathology, Hallym University Hangang Sacred Heart Hospital, Research Institute for Complementary and Alternative Medicine, Seoul, Korea. yhchoi@hallym.or.kr

Abstract

BACKGROUND
Although several studies have shown the role of interleukin-31 (IL-31) and its receptors in inducing pruritus in certain skin disorders, knowledge of its role in post-burn hypertrophic scars is insufficient. Therefore, the histopathological expression levels of IL-31, IL-31 receptor alpha (IL-31RA), and oncostatin M receptor (OSMR) in post-burn hypertrophic scar tissues were investigated and compared with normal tissue expression levels.
METHODS
Samples of hypertrophic scar tissue were obtained from 20 burn patients through punch biopsy. Normal samples were obtained from areas adjacent to the burn injury site of the same patients. Samples were placed in 10% neutral buffered formalin, embedded in paraplast, and processed into serial 5-μm sections. Immunohistochemistry results were semi-quantitatively evaluated for IL-31, IL-31RA, and OSMR. By hematoxylin and eosin staining, epidermal and dermal thickness were assessed with a microscope and digital camera. Intensities were rated on a scale of 1 to 4.
RESULTS
Percentages for IL-31, IL-31RA, and OSMR in the epidermal basal layer cell cytoplasm were significantly greater in the burn scar tissue compared to normal skin, as well as the dermal and epidermal thickness (p < .05). There was a significant difference in IL-31 epidermal basal layer intensity in burn scar tissue compared to normal skin (p < .05). Besides the OSMR basal layer intensity, IL-31 and IL-31RA intensities between the burn scar and normal tissues were not significant. However, correlations were significant, indicating that the greater the infiltration percentage, the higher the intensity (p < .05).
CONCLUSIONS
IL-31, IL-31RA, and OSMR expression levels are increased in hypertrophic scars compared with normal tissue.

Keyword

Burns; Cicatrix; Interleukin-31; IL31RA protein; OSMR protein

MeSH Terms

Biopsy
Burns
Cicatrix
Cicatrix, Hypertrophic*
Cytoplasm
Eosine Yellowish-(YS)
Formaldehyde
Hematoxylin
Humans
Immunohistochemistry
Pruritus
Receptors, Oncostatin M
Skin
Eosine Yellowish-(YS)
Formaldehyde
Hematoxylin
Receptors, Oncostatin M

Figure

  • Fig. 1. (A) Interleukin 31 (IL-31) control. (B) IL-31 scar. (C) IL-31 receptor alpha (IL-31RA) control. (D) IL-31RA scar. (E) Oncostatin M receptor (OSMR) control. (F) OSMR scar. IL-31, IL-31RA, and OSMR infiltration.

  • Fig. 2. Comparison of interleukin 31 (IL-31), IL-31 receptor alpha (IL-31RA), and oncostatin M receptor (OSMR) percentage values between the normal and scar tissue (n=20). *p<.05.


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