Ann Lab Med.  2018 Mar;38(2):165-168. 10.3343/alm.2018.38.2.165.

Incidental Identification of Plasmodium vivax During Routine Complete Blood Count Analysis Using the UniCel DxH 800

Affiliations
  • 1Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea. miziro@catholic.ac.kr
  • 2Division of Infectious Diseases, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Abstract

No abstract available.


MeSH Terms

Blood Cell Count*
Plasmodium vivax*
Plasmodium*

Figure

  • Fig. 1 Sizes of malaria signals (green population indicated by white arrow in 2D plot and black arrow in 3D plot) in nucleated red blood cell plot (rotated low angle light scatter [RLALS] vs axial light [AL2]) is roughly correlated with the parasite burden. (A and B) Positive: 9,134 parasites/µL on hospital day (HOD) 1. (C and D) Positive: 48 parasites/µL on HOD 3. (E and F) Negative: not found on HOD 7 in 2D and 3D plots.

  • Fig. 2 Results of thin peripheral blood smear and malaria PCR. (A) Morphology of different stages of the Plasmodium vivax (indicated by black arrows) in a thin peripheral blood smear. (B) Positive result (indicated by the red arrow) of electrophoresis after PCR screening for malaria.Abbreviations: SM, size marker; PC, positive control; NC, negative control.


Reference

1. Mace KE, Arguin PM. Malaria Surveillance - United States, 2014. MMWR Surveill Summ. 2017; 66:1–24.
2. Choi SJ, Park SW, Bae IG, Kim SH, Ryu SY, Kim HA, et al. Severe fever with thrombocytopenia syndrome in South Korea, 2013–2015. PLoS Negl Trop Dis. 2016; 10:e0005264. PMID: 28033338.
3. Lee HK, Kim SI, Chae H, Kim M, Lim J, Oh EJ, et al. Sensitive detection and accurate monitoring of Plasmodium vivax parasites on routine complete blood count using automatic blood cell analyzer (DxH800(TM)). Int J Lab Hematol. 2012; 34:201–207. PMID: 22074115.
4. Kimura M, Miyake H, Kim HS, Tanabe M, Arai M, Kawai S, et al. Species-specific PCR detection of malaria parasites by microtiter plate hybridization: clinical study with malaria patients. J Clin Microbiol. 1995; 33:2342–2346. PMID: 7494025.
5. Ciceron L, Jaureguiberry G, Gay F, Danis M. Development of a Plasmodium PCR for monitoring efficacy of antimalarial treatment. J Clin Microbiol. 1999; 37:35–38. PMID: 9854060.
6. Suh IB, Kim HJ, Kim JY, Lee SW, An SS, Kim WJ, et al. Evaluation of the Abbott Cell-Dyn 4000 hematology analyzer for detection and therapeutic monitoring of Plasmodium vivax in the Republic of Korea. Trop Med Int Health. 2003; 8:1074–1081. PMID: 14641842.
7. Fourcade C, Casbas MJ, Belaouni H, Gonzalez JJ, Garcia PJ, Pepio MA. Automated detection of malaria by means of the haematology analyser Coulter GEN.S. Clin Lab Haematol. 2004; 26:367–372. PMID: 15595992.
8. Sharma P, Bhargava M, Sukhachev D, Datta S, Wattal C. LH750 hematology analyzers to identify malaria and dengue and distinguish them from other febrile illnesses. Int J Lab Hematol. 2014; 36:45–55. PMID: 23773224.
9. Huh HJ, Oh GY, Huh JW, Chae SL. Malaria detection with the Sysmex XE-2100 hematology analyzer using pseudoeosinophilia and abnormal WBC scattergram. Ann Hematol. 2008; 87:755–759. PMID: 18427808.
10. McPherson RA, Pincus MR. Henry's Clinical Diagnosis and Management by Laboratory Methods E-Book. St. Louis, MO: Elsevier Health Sciences;2017.
11. Campuzano-Zuluaga G, Hanscheid T, Grobusch MP. Automated haematology analysis to diagnose malaria. Malar J. 2010; 9:346. PMID: 21118557.
12. Sharma S, Sethi N, Pujani M, Kushwaha S, Sehgal S. Abnormal WBC scattergram: a clue to the diagnosis of malaria. Hematology. 2013; 18:101–105. PMID: 22980408.
Full Text Links
  • ALM
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr