J Korean Acad Oral Health.  2017 Mar;41(1):3-8. 10.11149/jkaoh.2017.41.1.3.

Remineralization ability of fluoride varnish containing tricalcium phosphate by time

• Cho HJ
• Lee HC
• Lee JY
• Jin BH

• KMID: 2376032
• DOI: http://doi.org/10.11149/jkaoh.2017.41.1.3

Abstract

OBJECTIVES
The aim of this study was to evaluate the degree of remineralization over time after application of fluoride varnish with and without tricalcium phosphate (TCP).
METHODS
This in vitro study used extracted bovine lateral incisors without dental caries. Artificial lesions were created in the enamel specimens. The amount of mineral loss (ΔF(before)) was measured using quantitative light-induced fluorescence (QLF). Test fluoride varnishes (10 mg) were applied to the enamel surface of the specimen and dried for 4 min. No fluoride varnish was applied to the specimens in the control group. Each group was randomly assigned 12 specimens, and remineralization was allowed to occur to different time points (0.5, 1, 3, 6, 12, and 24 h) in each group. Specimens were washed with distilled water and dried with compressed air for 3 s. ΔF(after) was determined using QLF.
RESULTS
When fluoride varnish containing TCP was applied for up to 6 h, the amount of mineral loss significantly increased, and when non-TCP fluoride varnish was applied for up to 12 hours, the amount of mineral loss significantly increased (P<0.05). However, the amount of mineral loss was higher in the control group. The difference between ΔF(before) and ΔF(after) (ΔΔF) increased over time. There was a significant difference between the TCP group and the control group after 6 h. The non-TCP group showed a significant difference after 24 h compared to the control group. After 12 h, significant differences were observed in the TCP group compared to both the non-TCP and control groups.
CONCLUSIONS
This study showed that the degree of remineralization increased gradually over time after fluoride varnish application compared to the control group. In particular, fluoride varnish containing TCP showed better remineralization capability than varnish without TCP.

Keyword

Dental caries; Fluoride varnish; Remineralization; QLF; Time; Tricalcium phosphate

MeSH Terms

Compressed Air
Dental Caries
Dental Enamel
Fluorescence
Fluorides*
Fluorides, Topical
In Vitro Techniques
Incisor
Miners
Paint*
Water
Fluorides
Fluorides, Topical
Water

Figure

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  Fig. 1 The procedure of in-vitro experiment.

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  Fig. 2 The difference between ΔFbefore and ΔFafter (ΔΔF) by time. Tricalcium phosphate; TCP. ΔF means the amount of mineral loss measured by quantitative light-induced fluorescence (QLF™) between before and after fluoride varnish application. ΔΔF means the change amount of ΔF by time. *Different characters mean significant difference by Post hoc Tukey's test, P-value<0.05.

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