Yonsei Med J.  2016 Sep;57(5):1282-1285. 10.3349/ymj.2016.57.5.1282.

Platelet-Rich Fibrin Lysate Can Ameliorate Dysfunction of Chronically UVA-Irradiated Human Dermal Fibroblasts

• Wirohadidjojo YW
• Budiyanto
• Soebono H

• KMID: 2374177
• DOI: http://doi.org/10.3349/ymj.2016.57.5.1282

Abstract

To determine whether platelet-rich fibrin lysate (PRF-L) could restore the function of chronically ultraviolet-A (UVA)-irradiated human dermal fibroblasts (HDFs), we isolated and sub-cultured HDFs from six different human foreskins. HDFs were divided into two groups: those that received chronic UVA irradiation (total dosages of 10 J cm-2) and those that were not irradiated. We compared the proliferation rates, collagen deposition, and migration rates between the groups and between chronically UVA-irradiated HDFs in control and PRF-L-treated media. Our experiment showed that chronic UVA irradiation significantly decreased (p<0.05) the proliferation rates, migration rates, and collagen deposition of HDFs, compared to controls. Compared to control media, chronically UVA-irradiated HDFs in 50% PRF-L had significantly increased proliferation rates, migration rates, and collagen deposition (p<0.05), and the migration rates and collagen deposition of chronically UVA-irradiated HDFs in 50% PRF-L were equal to those of normal fibroblasts. Based on this experiment, we concluded that PRF-L is a good candidate material for treating UVA-induced photoaging of skin, although the best method for its clinical application remains to be determined.

Keyword

Collagen; cell migration; platelet-rich fibrin; cell proliferation; ultraviolet-A

MeSH Terms

Blood Platelets/*cytology/*metabolism
Cell Movement/radiation effects
Cell Proliferation/radiation effects
Cells, Cultured
Collagen/metabolism
Fibrin/*metabolism
Fibroblasts/*cytology/metabolism/*radiation effects
Humans
Skin/*cytology
Time Factors
Ultraviolet Rays/*adverse effects
Collagen
Fibrin