Restor Dent Endod.  2015 Feb;40(1):37-43. 10.5395/rde.2015.40.1.37.

Effects of applying antioxidants on bond strength of bleached bovine dentin

• Whang HJ
• Shin DH

• KMID: 2316922
• DOI: http://doi.org/10.5395/rde.2015.40.1.37

Abstract

OBJECTIVES
Some antioxidants are believed to restore dentin bond strength after dental bleaching. This study was done to evaluate the influence of antioxidants on the bond strength of bleached bovine dentin.
MATERIALS AND METHODS
Thirty incisors were randomly assigned to 10 groups (two unbleached control and eight bleached groups: immediate bonding IB, 4 wk delayed bonding DB, 10% sodium ascorbate treated SA, 10% alpha-tocopherol treated TP groups). Teeth in half of groups were subjected to thermal stress, whereas the remaining groups were not. Resin-dentin rods with a cross-sectional area of 2.25 mm2 were obtained and microtensile bond strength was determined at a crosshead speed of 1 mm/min. Fifteen specimens were prepared for SEM to compare the surface characteristics of each group. The change in dentin bond strength from thermal stress and antioxidant treatment was evaluated using two-way analysis of variance (ANOVA) and Sheffe's post hoc test at a significance level of 95%.
RESULTS
The control group exhibited the highest bond strength values, whereas IB group showed the lowest value before and after thermocycling. The DB group recovered its bond strength similar to that of the control group. The SA and TP groups exhibited similar bond strength values with those of the control and DB groups before thermocycling. However, The TP group did not maintain bond strength with thermal stress, whereas the SA group did.
CONCLUSIONS
Applying a 10% sodium ascorbate solution rather than 10% alpha-tocopherol solution for 60 sec is recommended to maintain dentin bond strength when restoring non-vitally bleached teeth.

Keyword

Antioxidant; Carbamide peroxide; Microtensile bond; Sodium ascorbate strength; Tooth bleaching; alpha-tocopherol

MeSH Terms

alpha-Tocopherol
Antioxidants*
Ascorbic Acid
Dentin*
Incisor
Tooth
Tooth Bleaching
Antioxidants
Ascorbic Acid
alpha-Tocopherol

Figure

• 

  Figure 1 Specimen preparation for microtensile bond strength test. (a) Access cavity preperation and non-vital bleaching; (b) Bonding substrate were restored with adhesive and composite to cavity wall; (c) Resin-dentin rods with a rectangular cross-sectional area of approximately 1.5 × 1.5 mm.

• 

  Figure 2 SEM photograph showing dentin surface treated with bleaching agent, antioxidants and without thermocycling. (a) Control group; (b) Immediate group; (c) 10% sodium ascorbate group; (d) 10% α-tocopherol group; (e) After 4 weeks group.

Reference

1. Lago AD, Garone-Netto N. Microtensile bond strength of enamel after bleaching. Indian J Dent Res. 2013; 24:104–109.

2. Nour El-din AK, Miller BH, Griggs JA, Wakefield C. Immediate bonding to bleached enamel. Oper Dent. 2006; 31:106–114.

3. Dishman MV, Covey DA, Baughan LW. The effects of peroxide bleaching on composite to enamel bond strength. Dent Mater. 1994; 10:33–36.

4. Lai SC, Tay FR, Cheung GS, Mak YF, Carvalho RM, Wei SH, Toledano M, Osorio R, Pashley DH. Reversal of compromised bonding in bleached enamel. J Dent Res. 2002; 81:477–481.

5. Efeoglu N, Wood D, Efeoglu C. Microcomputerised tomography evaluation of 10% carbamide peroxide applied to enamel. J Dent. 2005; 33:561–567.

6. Lewinstein I, Fuhrer N, Churaru N, Cardash H. Effect of different peroxide bleaching regimens and subsequent fluoridation on the hardness of human enamel and dentin. J Prosthet Dent. 2004; 92:337–342.

7. Lai SC, Mak YF, Cheung GS, Osorio R, Toledano M, Carvalho RM, Tay FR, Pashley DH. Reversal of compromised bonding to oxidized etched dentin. J Dent Res. 2001; 80:1919–1924.

8. Cavalli V, Reis AF, Giannini M, Ambrosano GM. The effect of elapsed time following bleaching on enamel bond strength of resin composite. Oper Dent. 2001; 26:597–602.

9. Titley KC, Torneck CD, Smith DC, Chernecky R, Adibfar A. Scanning electron microscopy observations on the penetration and structure of resin tags in bleached and unbleached bovine enamel. J Endod. 1991; 17:72–75.

10. Bulut H, Kaya AD, Turkun M. Tensile bond strength of brackets after antioxidant treatment on bleached teeth. Eur J Orthod. 2005; 27:466–471.

11. Kaya AD, Türkün M. Reversal of dentin bonding to bleached teeth. Oper Dent. 2003; 28:825–829.

12. Türkün M, Kaya AD. Effect of 10% sodium ascorbate on the shear bond strength of composite resin to bleached bovine enamel. J Oral Rehabil. 2004; 31:1184–1191.

13. Theriault A, Chao JT, Wang Q, Gapor A, Adeli K. Tocotrienol: a review of its therapeutic potential. Clin Biochem. 1999; 32:309–319.

14. Burton GW, Ingold KU. Vitamin E as an in vitro and in vivo antioxidant. Ann N Y Acad Sci. 1989; 570:7–22.

15. Cross CE, Halliwell B, Borish ET, Pryor WA, Ames BS, Saul RL, McCord JM, Harman D. Oxygen radicals and human disease. Ann Intern Med. 1987; 107:526–545.

16. Schilke R, Bauss O, Lisson JA, Schuckar M, Geurtsen W. Bovine dentin as a substitute for human dentin in shear bond strength measurements. Am J Dent. 1999; 12:92–96.

17. Reeves GW, Fitchie JG, Hembree JH Jr, Puckett AD. Microleakage of new dentin bonding systems using human and bovine teeth. Oper Dent. 1995; 20:230–235.

18. Camargo MA, Marques MM, de Cara AA. Morphological analysis of human and bovine dentine by scanning electron microscope investigation. Arch Oral Biol. 2008; 53:105–108.

19. Schilke R, Lisson JA, Bauss O, Geurtsen W. Comparison of the number and diameter of dentinal tubules in human and bovine dentine by scanning electron microscopic investigation. Arch Oral Biol. 2000; 45:355–361.

20. Attin T, Vollmer D, Wiegand A, Attin R, Betke H. Subsurface microhardness of enamel and dentin after different external bleaching procedures. Am J Dent. 2005; 18:8–12.

21. Güler E, Gönülol N, Özyilmaz ÖY, Yücel AÇ. Effect of sodium ascorbate on the bond strength of silorane and methacrylate composites after vital bleaching. Braz Oral Res. 2013; 27:299–304.

22. Smit MJ, Anderson R. Biochemical mechanisms of hydrogen peroxide- and hypochlorous acid-mediated inhibition of human mononuclear leukocyte functions in vitro: protection and reversal by anti-oxidants. Agents Actions. 1992; 36:58–65.

23. Garcia EJ, Oldoni TL, Alencar SM, Reis A, Loguercio AD, Grande RH. Antioxidant activity by DPPH assay of potential solutions to be applied on bleached teeth. Braz Dent J. 2012; 23:22–27.

24. Elisia I, Kitts DD. Quantification of hexanal as an index of lipid oxidation in human milk and association with antioxidant components. J Clin Biochem Nutr. 2011; 49:147–152.

25. Brigelius-Flohé R, Traber MG. Vitamin E: function and metabolism. FASEB J. 1999; 13:1145–1155.

26. Sasaki RT, Flório FM, Basting RT. Effect of 10% sodium ascorbate and 10% alpha-tocopherol in different formulations on the shear bond strength of enamel and dentin submitted to a home-use bleaching treatment. Oper Dent. 2009; 34:746–752.

27. Buettner GR. The pecking order of free radicals and antioxidants: lipid peroxidation, alpha-tocopherol, and ascorbate. Arch Biochem Biophys. 1993; 300:535–543.

28. Kum KY, Lim KR, Lee CY, Park KH, Safavi KE, Fouad AF, Spångberg LS. Effects of removing residual peroxide and other oxygen radicals on the shear bond strength and failure modes at resin-tooth interface after tooth bleaching. Am J Dent. 2004; 17:267–270.

29. Perdigão J, Francci C, Swift EJ Jr, Ambrose WW, Lopes M. Ultra-morphological study of the interaction of dental adhesives with carbamide peroxide-bleached enamel. Am J Dent. 1998; 11:291–301.